首页|A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus∞

A transcriptional cascade involving BBX22 and HY5 finely regulates both plant height and fruit pigmentation in citrus∞

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Dwarfing is a pivotal agronomic trait affecting both yield and quality.Citrus species exhibit substantial variation in plant height,among which internode length is a core element.However,the molecular mechanism governing internode elongation remains unclear.Here,we unveiled that the transcriptional cascade con-sisting of B-BOX DOMAIN PROTEIN 22(BBX22)and ELONGATED HYPOCOTYL 5(HY5)finely tunes plant height and internode elongation in citrus.Loss-of-function mutations of BBX22 in an early-flowering citrus(Citrus hindsii"SJG")promoted internode elongation and reduced pigment accumulation,whereas ectopic ex-pression of BBX22 in SJG,sweet orange(C.sinensis),pomelo(C.maxima)or heterologous expression of BBX22 in tomato(Solanum lyco-persicum)significantly decreased internode length.Furthermore,exogenous application of gibberellin A3(GA3)rescued the shortened in-ternode and dwarf phenotype caused by BBX22 overexpression.Additional experiments re-vealed that BBX22 played a dual role in regu-lation internode elongation and pigmentation in citrus.On the one hand,it directly bound to and activated the expression of HY5,GA metabo-lism gene(GA2 OXIDASE 8,GA2ox8),car-otenoid biosynthesis gene(PHYTOENE SYN-THASE 1,PSY1)and anthocyanin regulatory gene(Ruby1,a MYB DOMAIN PROTEIN).On the other hand,it acted as a cofactor of HY5,en-hancing the ability of HY5 to regulate target genes expression.Together,our results reveal the critical role of the transcriptional cascade consisting of BBX22 and HY5 in controlling in-ternode elongation and pigment accumulation in citrus.Unraveling the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.

anthocyaninsBBX22carotenoidscitrusHY5internode elongationplant height

Jialing Fu、Li Liao、Jiajing Jin、Zhihao Lu、Juan Sun、Lizhi Song、Yue Huang、Shengjun Liu、Ding Huang、Yuantao Xu、Jiaxian He、Bin Hu、Yiqun Zhu、Fangfang Wu、Xia Wang、Xiuxin Deng、Qiang Xu

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National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China

Hubei Hongshan Laboratory,Wuhan 430070,China

National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops/College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China

Science and Technology Innovation Research Center of Majia Pomelo,Shangrao 334000,China

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2024

10.1111/jipb.13719

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)