首页|The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs∞

The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs∞

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Pathogens generate and secrete effector proteins to the host plant cells during pathogenesis to promote virulence and colonization.If the plant carries resist-ance(R)proteins that recognize pathogen effectors,effector-triggered immunity(ETI)is activated,re-sulting in a robust immune response and hyper-sensitive response(HR).The bipartite effector AvrRps4 from Pseudomonas syringae pv.pisi has been well studied in terms of avirulence function.In planta,AvrRps4 is processed into two parts.The C-terminal fragment of AvrRps4(AvrRps4C)induces HR in tumip and is recognized by the paired resistance proteins AtRRS1/AtRPS4 in Arabidopsis.Here,we show that AvrRps4C targets a group of Arabidopsis WRKY,including WRKY46,WRKY53,WRKY54,and WRKY70,to induce its virulence function.Indeed,AvrRps4C suppresses the general binding and tran-scriptional activities of immune-positive regulator WRKY54 and WRKY54-mediated resistance.AvrRps4C interferes with WRKY54's binding activity to target gene SARD1 in vitro,suggesting WRKY54 is sequestered from the SARD1 promoter by AvrRps4C.Through the interaction of AvrRps4C with four WRKYs,AvrRps4 enhances the formation of homo-/heterotypic complexes of four WRKYs and seques-ters them in the cytoplasm,thus inhibiting their function in plant immunity.Together,our results provide a detailed virulence mechanism of AvrRps4 through its C-terminus.

AvrRps4bacterial effectoreffector-triggered immunityimmune response suppressiontranscription factorWRKY

Quang-Minh Nguyen、Arya Bagus Boedi Iswanto、Hobin Kang、Jiyun Moon、Kieu Anh Thi Phan、Geon Hui Son、Mi Chung Suh、Eui-Hwan Chung、Walter Gassmann、Sang Hee Kim

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Division of Applied Life Science(BK21 Four Program),Plant Molecular Biology and Biotechnology Research Center,Gyeongsang National University,Jinju 52828,Korea

Department of Life Science,Sogang University,Seoul 04107,Korea

Department of Plant Biotechnology,Korea University,Seoul 02841,Korea

Division of Plant Science and Technology,Christopher S.Bond Life Sciences Center and Interdisciplinary Plant Group,University of Missouri,Columbia,Missouri 65211,USA

Division of Life Science and Research Institute of Molecular Alchemy,Gyeongsang National University,Jinju 52828,Korea

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2024

10.1111/jipb.13710

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)