首页|ICE1 interacts with IDD14 to transcriptionally activate QQS to increase pollen germination and viability

ICE1 interacts with IDD14 to transcriptionally activate QQS to increase pollen germination and viability

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In flowering plants,sexual reproductive success depends on the production of viable pollen grains.However,the mechanisms by which QUA QUINE STARCH(QQS)regulates pollen development and how transcriptional activators facilitate the transcription of QQS in this process remain poorly understood.Here,we demonstrate that INDUCER OF CBF EXPRESSION 1(ICE1),a basic helix-loop-helix(bHLH)transcription factor,acts as a key transcriptional activator and positively regulates QQS expression to increase pollen germination and viability in Arabidopsis thaliana by interacting with INDETERMINATE DOMAIN14(IDD14).In our genetic and biochemical experiments,over-expression of ICE1 greatly promoted both the ac-tivation of QQS and high pollen viability mediated by QQS.IDD14 additively enhanced ICE1 function by promoting the binding of ICE1 to the QQS pro-moter.In addition,mutation of ICE1 significantly repressed QQS expression;the impaired function of QQS and the abnormal anther dehiscence jointly affected pollen development of the ice1-2 mutant.Our results also showed that the enhancement of pollen activity by ICE1 depends on QQS.Furthermore,QQS interacted with CUT1,the key enzyme for long-chain lipid biosynthesis.This interaction both promoted CUT1 activity and regulated pollen lipid metabolism,ultimately de-termining pollen hydration and fertility.Our results not only provide new insights into the key function of QQS in promoting pollen development by regu-lating pollen lipid metabolism,but also elucidate the mechanism that facilitates the transcription of QQS in this vital developmental process.

CUT1ICE1IDD14pollen germination and viabilityQQS

Landi Luo、Yan Zheng、Xieshengyang Li、Qian Chen、Danni Yang、Zhijia Gu、Ya Yang、Yunqiang Yang、Xiangxiang Kong、Yongping Yang

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CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,The Chinese Academy of Sciences,Xishuangbanna 666303,China

Germplasm Bank of Wild Species,Yunnan Key Laboratory for Crop Wild Relatives Omics,Kunming Institute of Botany,The Chinese Academy of Sciences,Kunming 650201,China

School of Agriculture,Yunnan University,Kunming 650091,China

Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,The Chinese Academy of Sciences,Kunming 650201,China

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2024

10.1111/jipb.13725

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(8)