Abstract
Although the cell membrane and cytoskeleton play essential roles in cellular morphogenesis,the inter-action between the membrane and cytoskeleton is poorly understood.Cotton fibers are extremely elongated single cells,which makes them an ideal model for studying cell development.Here,we used the sphingolipid biosynthesis inhibitor,fumonisin B1(FB1),and found that it effectively suppressed the myeloblastosis(MYB)transcription factor GhMYB86,thereby negatively affecting fiber elongation.A direct target of GhMYB86 is GhTUB7,which encodes the tubulin protein,the major component of the micro-tubule cytoskeleton.Interestingly,both the over-expression of GhMYB86 and GhTUB7 caused an ectopic microtubule arrangement at the fiber tips,and then leading to shortened fibers.Moreover,we found that GhMBE2 interacted with GhMYB86 and that FB1 and reactive oxygen species induced its transport into the nucleus,thereby enhancing the promotion of GhTUB7 by GhMYB86.Overall,we established a GhMBE2-GhMYB86-GhTUB7 regu-lation module for fiber elongation and revealed that membrane sphingolipids affect fiber elongation by altering microtubule arrangement.
基金项目
National Natural Science Foundation of China(31571722)
National Natural Science Foundation of China(31971984)
Genetically Modified Organisms Breeding Major Project of China(2018ZX0800921B)
Fundamental Research Funds for the Central Universities(SWU-XDJH202315)
维普
万方数据