Abstract
NAC transcription factors(TFs)are pivotal in plant immunity against diverse pathogens.Here,we re-port the functional and regulatory network of MNAC3,a novel NAC TF,in rice immunity.MNAC3,a transcriptional activator,negatively modulates rice immunity against blast and bacterial leaf blight diseases and pathogen-associated molecular pat-tem(PAMP)-triggered immune responses.MNAC3 binds to a CACG cis-element and activates the transcription of immune-negative target genes OsINO80,OsJAZ10,and OsJAZ11.The negative function of MNAC3 in rice immunity depends on its transcription of downstream genes such as OsINO80 and OsJAZ10.MNAC3 interacts with immunity-related OsPP2C41(a protein phospha-tase),ONAC066(a NAC TF),and OsDjA6(a DnaJ chaperone).ONAC066 and OsPP2C41 attenuate MNAC3 transcriptional activity,while OsDjA6 pro-motes it.Phosphorylation of MNAC3 at S163 is critical for its negative functions in rice immunity.OsPP2C41,which plays positive roles in rice blast resistance and chitin-triggered immune responses,dephosphorylates MNAC3,suppressing its tran-scriptional activity on the target genes OsINO80,OsJAZ10,and OsJAZ11 and promoting the trans-location of MNAC3 from nucleus to cytoplasm.These results establish a MNAC3-centered regu-latory network in which OsPP2C41 dephosphor-ylates MNAC3,attenuating its transcriptional activity on downstream immune-negative target genes in rice.Together,these findings deepen our under-standing of molecular mechanisms in rice immunity and offer a novel strategy for genetic improvement of rice disease resistance.
基金项目
National Natural Science Foundation of China(32072403)
National Natural Science Foundation of China(31871945)
National Key Research and Development Program of China(2016YFD0100600)
维普
万方数据