A NAC transcription factor MNAC3-centered regulatory network negatively modulates rice immunity against blast disease

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外文摘要：NAC transcription factors(TFs)are pivotal in plant immunity against diverse pathogens.Here,we re-port the functional and regulatory network of MNAC3,a novel NAC TF,in rice immunity.MNAC3,a transcriptional activator,negatively modulates rice immunity against blast and bacterial leaf blight diseases and pathogen-associated molecular pat-tem(PAMP)-triggered immune responses.MNAC3 binds to a CACG cis-element and activates the transcription of immune-negative target genes OsINO80,OsJAZ10,and OsJAZ11.The negative function of MNAC3 in rice immunity depends on its transcription of downstream genes such as OsINO80 and OsJAZ10.MNAC3 interacts with immunity-related OsPP2C41(a protein phospha-tase),ONAC066(a NAC TF),and OsDjA6(a DnaJ chaperone).ONAC066 and OsPP2C41 attenuate MNAC3 transcriptional activity,while OsDjA6 pro-motes it.Phosphorylation of MNAC3 at S163 is critical for its negative functions in rice immunity.OsPP2C41,which plays positive roles in rice blast resistance and chitin-triggered immune responses,dephosphorylates MNAC3,suppressing its tran-scriptional activity on the target genes OsINO80,OsJAZ10,and OsJAZ11 and promoting the trans-location of MNAC3 from nucleus to cytoplasm.These results establish a MNAC3-centered regu-latory network in which OsPP2C41 dephosphor-ylates MNAC3,attenuating its transcriptional activity on downstream immune-negative target genes in rice.Together,these findings deepen our under-standing of molecular mechanisms in rice immunity and offer a novel strategy for genetic improvement of rice disease resistance.

外文关键词：

NAC transcription factor MNAC3OsINO80OsJAZ10OsJAZ11protein phosphatase OsPP2C41rice immunity

作者：

Hui Wang、Yan Bi、Yuqing Yan、Xi Yuan、Yizhou Gao、Muhammad Noman、Dayong Li、Fengming Song

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作者单位：

National Key Laboratory for Rice Biology and Breeding,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China

Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China

College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China

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基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of China

项目编号：

32072403318719452016YFD0100600

出版年：

2024

DOI：

10.1111/jipb.13727

植物学报(英文版)