The METHYLTRANSFERASE B-SERRATE interaction mediates the reciprocal regulation of microRNA biogenesis and RNA m6A modification

Haiyan Bai ¹Yanghuan Dai ¹Panting Fan ²Yiming Zhou ¹Xiangying Wang ¹Jingjing Chen ¹Yuzhe Jiao ¹Chang Du ¹Zhuoxi Huang ¹Yuting Xie ¹Xiaoyu Guo ¹Xiaoqiang Lang ³Yongqing Ling ⁴Yizhen Deng ⁵Qi Liu ³Shengbo He ²Zhonghui Zhang¹

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作者信息

1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Science,South China Normal University,Guangzhou 510631,China
2. Guangdong Laboratory for Lingnan Modern Agriculture,State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou 510642,China
3. Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Guangzhou 510640,China;Guangdong Key Laboratory of New Technology in Rice Breeding,Guangdong Rice Engineering Laboratory,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
4. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Science,South China Normal University,Guangzhou 510631,China;Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Guangzhou 510640,China;Guangdong Key Laboratory of New Technology in Rice Breeding,Guangdong Rice Engineering Laboratory,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou
5. Guangdong Province Key Laboratory of Microbial Signals and Disease,State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou 510642,China
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Abstract

In eukaryotes,RNA N6-methyladenosine(m6A)modification and microRNA(miRNA)-mediated RNA silencing represent two critical epigenetic regulatory mechanisms.The m6A methyl-transferase complex(MTC)and the micro-processor complex both undergo liquid-liquid phase separation to form nuclear membraneless organelles.Although m6A methyltransferase has been shown to positively regulate miRNA biogenesis,a mechanism of reciprocal regu-lation between the MTC and the microprocessor complex has remained elusive.Here,we demonstrate that the MTC and the micro-processor complex associate with each other through the METHYLTRANSFERASE B(MTB)-SERRATE(SE)interacting module.Knockdown of MTB impaired miRNA biogenesis by diminishing microprocessor complex binding to primary miRNAs(pri-miRNAs)and their re-spective MIRNA loci.Additionally,loss of SE function led to disruptions in transcriptome-wide m6A modification.Further biochemical assays and fluorescence recovery after photobleaching(FRAP)assay indicated that SE enhances the liquid-liquid phase separation and solubility of the MTC.Moreover,the MTC exhibited en-hanced retention on chromatin and diminished binding to its RNA substrates in the se mutant background.Collectively,our results reveal the substantial regulatory interplay between RNA m6A modification and miRNA biogenesis.

Key words

liquid-liquid phase separation/microRNA biogenesis/MTB/RNA m6A modification/SE

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出版年

2024

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCDCSCD

影响因子：0.921

ISSN：1672-9072

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