The OsMAPK5-OsWRKY72 module negatively regulates grain length and grain weight in rice

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外文摘要：Grain size and grain weight are important de-terminants for grain yield.In this study,we identify a novel OsMAPK5-OsWRKY72 module that negatively regulates grain length and grain weight in rice.We found that loss-of-function of OsMAPK5 leads to larger cell size of the rice spikelet hulls and a significant increase in both grain length and grain weight in an indica variety Minghui 86(MH86).OsMAPK5 interacts with OsMAPKK3/4/5 and OsWRKY72 and phosphor-ylates OsWRKY72 at T86 and S88.Similar to the osmapk5 MH86 mutants,the oswrky72 knockout MH86 mutants exhibited larger size of spikelet hull cells and increased grain length and grain weight,whereas the OsWRKY72-overexpression MH86 plants showed opposite phenotypes.OsWRKY72 targets the W-box motifs in the promoter of OsARF6,an auxin response factor involved in auxin signaling.Dual-luciferase re-porter assays demonstrated that OsWRKY72 activates OsARF6 expression.The activation effect of the phosphorylation-mimicking OsWR-KY72T86D/S88D on OsARF6 expression was sig-nificantly enhanced,whereas the effects of the OsWRKY72 phosphorylation-null mutants were significantly reduced.In addition,auxin levels in young panicles of the osmapk5 and oswrky72 mutants were significantly higher than that in the wild-type MH86.Collectively,our study un-covered novel connections of the OsMAPKK 3/4/5-OsMAPK5-mediated MAPK signaling,OsWRKY72-mediated transcription regulation,and OsARF6-mediated auxin signaling pathways in regulating grain length and grain weight in an indica-type rice,providing promising targets for molecular breeding of rice varieties with high yield and quality.

外文关键词：

grain lengthgrain weightOsARF6OsMAPK5OsWRKY72rice

作者：

Fuxiang Wang、Jiexin Lin、Fan Yang、Xiaofeng Chen、Yiyi Liu、Lingnan Yan、Jing Chen、Zonghua Wang、Huaan Xie、Jianfu Zhang、Huibin Xu、Songbiao Chen

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作者单位：

Marine and Agricultural Biotechnology Laboratory,College of Geography and Oceanography,Minjiang University,Fuzhou 350108,China

National Rice Engineering Laboratory of China,Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China

College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China

College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China

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出版年：

2024

DOI：

10.1111/jipb.13786

植物学报(英文版)

中国植物学会

植物学报(英文版)

CSTPCD

影响因子：0.921

ISSN：1672-9072

年,卷(期)：2024.66(12)