首页|Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice

Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice

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Sucrose functions as a signaling molecule in several metabolic pathways as well as in various devel-opmental processes.However,the molecular mech-anisms by which sucrose regulates these processes remain largely unknown.In the present study,we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice.Exogenous application of sucrose promoted flowering by inducing florigen gene expression.Reduction of sucrose levels in the phloem through genetic modifications,such as the overexpression of the vacuolar invertase OsVIN2 or the mutation of OsSUT2,a sucrose transporter,de-layed flowering.Analysis of relative transcript levels of floral regulatory genes showed that sucrose acti-vated Ehd1 upstream of the florigen,with no sig-nificant effect on the expression of other upstream genes.Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded.The Ghd7 protein interacted with the E3 ligase IPA INTER-ACTING PROTEIN1(IPI1),and sucrose-induced K48-linked polyubiquitination of Ghd7 via IPI1,leading to protein degradation.Mutants defective in IPI1 de-layed flowering,confirming its role in modulating proteins involved in flowering.We conclude that su-crose acts as a signaling molecule to induce flow-ering by promoting Ghd7 degradation via IPI1.

flowering timeGhd7IPI1K48-linked poly-ubiquitinationricesucrose

Lae-Hyeon Cho、Jinmi Yoon、Gibeom Baek、Win Tun、Hyeok Chan Kwon、Dae-Woo Lee、Seok-Hyun Choi、Yang-Seok Lee、Jong-Seong Jeon、Gynheung An

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Department of Plant Bioscience,College of Natural Resources and Life Science,Pusan National University,Miryang 50463,Korea

Life and Industry Convergence Research Institute,Pusan National University,Miryang 50463,Korea

Department of Biological Sciences,Inha University,Incheon 22212,Korea

Department of Biological Sciences and Bioengineering,Inha University/Industry-Academia Interactive R&E Center for Bioprocess Innovation,Inha University,Incheon 22212,Korea

Crop Biotech Institute and Graduate School of Green-Bio Science,Kyung Hee University,Yongin 17104,Korea

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2024

10.1111/jipb.13790

植物学报(英文版)
中国植物学会

植物学报(英文版)

CSTPCD
影响因子：0.921
ISSN：1672-9072
年,卷(期)：2024.66(12)