An in vitro rapid propagation system using scale buds of Itoh hybrids'He Xie'can overcome the shortcomings of the slow traditional breeding methods and promote the adoption of the excellent Itoh hybrid varieties.In this study,we used the buds of'He Xie'as explants to investigate the effects of various factors including disinfection time,plant growth regulators(PGRs)concentration and root induction time,and different rooted seedling grades on the initiation,prolifera-tion,rooting and domestication of'He Xie'with one-way experimental design.Our results showed:the optimal disinfection time of buds by 2％sodium hypochlorite solution was 12 min,with a contamination rate of 9.09％;the optimal initial culture medium was MS+1.5 mg·L-1 6-BA+0.2 mg·L-1 GA3+0.5 mg·L-1 AgNO3;the optimal proliferation culture medium was MS+450 mg·L-1 CaCl2+0.5 mg·L-1 6-BA+0.2 mg·L-1 IBA+0.2 mg·L-1 GA3+0.5 mg·L-1 AgNO3 with a proliferation rate of 3.3.Rootless seedlings were cultured on root induction medium 1/2MS+1.0 mg·L-1 putrescine+2.0 mg·L-1 IBA for 8 d at 4℃ in dark and then 30 d at room temperature under light,and finally transferred to root formation medium 1/2MS+1.0 g·L-1 AC for 20 d,a rooting rate of 66.7％was observed.The rooted seedlings were transplanted on a growing matrix of per-lite:vermiculite:charcoal soil=1:1:1(v/v/v)for 60 d,with the highest transplant survival rate of 52.0％being observed for first-grade rooted seedlings,but most of the second-and third-grade seedlings being dead,suggesting that the quality of rooting is critical for transplant survival.
Itoh'He Xie'initiation and proliferationrootingscale budstransplant domestication
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