拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响
Effect of Amino Acid Point Mutations on the Structure and Func-tion of Phytochrome B in Arabidopsis thaliana
陈艳晓 1李亚萍 2周晋军 3解丽霞 2彭永彬 2孙伟 2和亚男 2蒋聪慧 2王增兰 4郑崇珂 2谢先芝2
作者信息
- 1. 山东师范大学生命科学学院,济南 250014;山东省农业科学院湿地农业与生态研究所,济南 250100
- 2. 山东省农业科学院湿地农业与生态研究所,济南 250100
- 3. 山东省农业科学院农作物种质资源研究所,济南 250100
- 4. 山东师范大学生命科学学院,济南 250014
- 折叠
摘要
生物体为适应外界不断变化的光环境,进化出不同的光受体,其中光敏色素是一类经典的植物感受红光和远红光的受体蛋白,其通过暗适应的Pr状态和光激活的Pfr状态之间的光转换来检测红光和远红光.植物光敏色素具有1个保守的N端感光区域和1个C端调节区域,其中N端部分包括NTE、PAS、GAF和PHY亚结构域,C端部分包括2个PAS结构域和1个组氨酸激酶相关结构域(HKRD).为深入了解光敏色素的结构及功能,已获得许多光敏色素功能缺失或氨基酸位点突变体,并对其进行功能研究,发现N端结构域在光敏色素的光谱特性、光信号感知和光信号转导等方面均具有重要作用;而C端结构域是光敏色素的二聚化与核定位所必需.该文综述了拟南芥(Arabidopsis thaliana)中光敏色素B(phyB)各亚结构域中氨基酸位点突变对其功能的影响,以期深入理解phyB的结构及功能,为未来通过基因编辑手段进行作物农艺性状的遗传改良奠定基础.
Abstract
Organisms have evolved different photoreceptors to adapt to the ever-changing conditions of the external light environment.Phytochromes(phys)are one of the classic plant photoreceptors,mainly perceiving red and far-red light.Phytochromes detect red and far-red light through the light conversion between the dark-adapted Pr state and the light-activated Pfr state.All plant phytochromes have a conserved N-terminal photoreceptor region and a C-terminal regulatory region.The N-terminal includes NTE,PAS,GAF,and PHY subdomains,while C-terminal includes two PAS domains and a histidine kinase-related domain(HKRD).In order to understand how the structure of photochromes con-trols its function,many function-deficient photochrome derivatives and amino acid point mutants have been obtained and studied.The N-terminal domain plays important roles in the spectral properties,light signal perception and light signal transduction of phyB.The C-terminal domain is essential for dimerization and nuclear localization of photochrome.This paper mainly reviews point mutations of amino acid in various subdomains of phyB in Arabidopsis thaliana and their ef-fects on the function of phyB,in order to have a better understanding of the structure and functional regulation of phyB.It lays a foundation for obtaining crops with desired agronomic characteristics through gene editing.
关键词
光敏色素B/拟南芥/结构域/氨基酸位点突变/功能Key words
phyB/Arabidopsis thaliana/domain/amino acid point mutation/function引用本文复制引用
基金项目
国家自然科学基金(32070216)
山东省农科院农业科技创新工程项目(CXGC2023A15)
出版年
2024
国家科技期刊平台
NSTL
万方数据