摘要
以2个不同基因型的捕虫堇圆切捕虫堇(Pinguicula cyclosecta)和塞提捕虫堇(P.'Sethos')叶片为外植体,通过探究不定芽再生的影响因素,成功建立了捕虫堇高效再生体系.结果表明,2种捕虫堇对消毒方式和基本培养基种类要求相同,但再生能力有显著差异.圆切捕虫堇叶片在MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA培养基上再生能力最强,再生率为92.22％,再生系数达4.84.在MS+0.6 mg·L-1 6-BA+0.1 mg·L-1 NAA培养基上,不定芽增殖系数达4.98.塞提捕虫堇叶片在MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA培养基中再生率最高,为77.78％,再生系数为6.12,在增殖培养基MS+0.3 mg·L-16-BA+0.1 mg·L-1 NAA上,增殖系数可达4.84.2种捕虫堇在1/2MS+0.1 mg·L-1 IBA培养基上进行生根培养,根系生长状态最佳.该研究解决了捕虫堇繁殖系数低及工厂化育苗难等问题,为其规模化生产和育种改良提供了技术支持.
Abstract
Using two different genotypes of Pinguicula cyclosecta and P.'Sethos'as explants,an efficient system for adventitious bud regeneration of Pinguicula was established by studying the factors affecting regeneration.The results showed that the two kinds of Pinguicula had the same requirements for the disinfection scheme and basic medium,but the regeneration ability differed.The best medium for P.cyclosecta leaf regeneration was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 92.22％and a regeneration coefficient was 4.84.On MS+0.6 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient of adventitious buds reached 4.98.The best medium for the leaf regeneration of P.'Sethos'was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 77.78％and a regeneration coefficient of 6.12.On the MS+0.3 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient reached 4.84.After root culture(1/2MS+0.1 mg·L-1 IBA),the root state of the two kinds of Pinguicula was the best.This study solved the problems of a low reproduction coefficient and difficulty in factory breeding of Pinguicula and provided technical support for its large-scale production and breeding improvement.
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