Establishment of an Efficient Leaf Regeneration System for Pinguicula
Using two different genotypes of Pinguicula cyclosecta and P.'Sethos'as explants,an efficient system for adventitious bud regeneration of Pinguicula was established by studying the factors affecting regeneration.The results showed that the two kinds of Pinguicula had the same requirements for the disinfection scheme and basic medium,but the regeneration ability differed.The best medium for P.cyclosecta leaf regeneration was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 92.22％and a regeneration coefficient was 4.84.On MS+0.6 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient of adventitious buds reached 4.98.The best medium for the leaf regeneration of P.'Sethos'was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,with a regeneration rate of 77.78％and a regeneration coefficient of 6.12.On the MS+0.3 mg·L-1 6-BA+0.1 mg·L-1 NAA media,the proliferation coefficient reached 4.84.After root culture(1/2MS+0.1 mg·L-1 IBA),the root state of the two kinds of Pinguicula was the best.This study solved the problems of a low reproduction coefficient and difficulty in factory breeding of Pinguicula and provided technical support for its large-scale production and breeding improvement.
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