摘要
水稻(Oryza sativa)是世界上最重要的粮食作物之一.提高水稻抗氧化性,进而提高抗逆性是保障其高产稳产的重要途径.选用籼稻华占(HZ)和粳稻热研2号(Nekken2)及以其为亲本构建的120个重组自交系(RILs),分别在分蘖期、灌浆期和成熟期测定亲本及其后代剑叶、颖壳及籽粒中羟基自由基清除率、总酚含量、黄酮含量和花青苷含量,同时基于已构建的高密度遗传连锁图谱进行数量性状基因座(QTL)定位.结果共挖掘到62个与水稻抗氧化损伤有关的QTLs,其中LOD值最高达4.36.对这些QTL区间内相关候选基因的表达进行定量分析,结果表明LOC_Os06g01850、LOC_Os12g07820、LOC_Os12g07830和LOC_Os03g60509等13个基因的表达在不同时期的双亲间差异显著.研究挖掘到众多与水稻抗氧化性相关的QTLs,为进一步定位并克隆相关基因,选育抗性强且营养价值高的水稻新品种奠定基础.
Abstract
Rice(Oryza sativa)is one of the most important food crops in the world.Improving its antioxidant ability and stress resistance is an important way to ensure high and stable yields.In this study,we used the indica rice HZ and the japonica rice Nekken2 as parents and the 120 recombinant inbred line population constructed from them as experimental materials to determine the hydroxyl radical scavenging rate,total phenol content,flavonoid content,and anthocyanin content in sword leaves,glume shells and grains of parents and their progeny at three stages:the tillering stage,the grain filling stage and the maturity stage.Additionally,a total of 62 QTLs related to rice antioxidant damage were identified on the basis of the constructed high-density genetic map for QTL mapping,with an LOD value of up to 4.36.A quantitative analysis of candidate genes related to antioxidant damage ability in these regions revealed that thirteen candidate genes,including LOC_Os06g01850,LOC_Os12g07820,LOC_Os12g07830,and LOC_Os03g60509 were significantly differen-tially expressed between the two parents at different growth stages.A multitude of QTLs associated with antioxidant damage resistance in rice were identified,providing a foundation for further mapping and cloning of related genes and the development of new rice varieties with increased resistance and nutritional value.
基金项目
浙江省自然科学基金重点项目(LZ23C130003)
2024年国家级大学生创新创业训练计划项目()
2024年浙江省大学生科技创新活动计划暨新苗人才计划()
国家科技期刊平台
NSTL
万方数据