Genetic Locus Mining and Candidate Gene Analysis of Antioxidant Traits in Rice
Rice(Oryza sativa)is one of the most important food crops in the world.Improving its antioxidant ability and stress resistance is an important way to ensure high and stable yields.In this study,we used the indica rice HZ and the japonica rice Nekken2 as parents and the 120 recombinant inbred line population constructed from them as experimental materials to determine the hydroxyl radical scavenging rate,total phenol content,flavonoid content,and anthocyanin content in sword leaves,glume shells and grains of parents and their progeny at three stages:the tillering stage,the grain filling stage and the maturity stage.Additionally,a total of 62 QTLs related to rice antioxidant damage were identified on the basis of the constructed high-density genetic map for QTL mapping,with an LOD value of up to 4.36.A quantitative analysis of candidate genes related to antioxidant damage ability in these regions revealed that thirteen candidate genes,including LOC_Os06g01850,LOC_Os12g07820,LOC_Os12g07830,and LOC_Os03g60509 were significantly differen-tially expressed between the two parents at different growth stages.A multitude of QTLs associated with antioxidant damage resistance in rice were identified,providing a foundation for further mapping and cloning of related genes and the development of new rice varieties with increased resistance and nutritional value.
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