首页|拟南芥光敏色素突变体种子萌发的光温敏感性

拟南芥光敏色素突变体种子萌发的光温敏感性

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为阐明光敏色素家族基因突变对拟南芥(Arabidopsis thaliana)种子萌发时环境适应性的影响,以野生型(Col-0)种子为对照,对比了phyA、phyB、phyC、phyD和phyE单突变体种子在12种光温环境下萌发率的差异.结果表明,phyA突变体种子适应在红光下萌发,而不适应在远红光和高温(35℃)下萌发.phyB突变体种子适应在白光和远红光背景下的低温(15℃)和适温(25℃)下萌发;而不适应在高温(35℃)下萌发.phyC突变体种子适应在除白光35℃以外的11种光温环境下萌发.phyD或phyE突变体种子适应在低温(15℃)和适温(25℃)下萌发,而不适应在高温(35℃)下萌发;适应在红光和白光下萌发,而不适应在黑暗和远红光下萌发.5个成员中,phyB、phyC和phyD的突变体种子可能丧失光温整合能力,而phyA和phyE的突变体种子未丧失光温整合能力.综上,光敏色素家族基因突变导致种子萌发对光、温和光×温的适应性改变,通过靶向修饰光敏色素家族基因可提升种子萌发对不同生态环境的适应性.
Photothermal Sensitivity of Phytochrome Mutants During Seed Germination in Arabidopsis thaliana
The phytochrome gene family play a critical role in mediating photothermal responses during Arabidopsis thaliana seed germination.Here we evaluated the germination rates of phyA,phyB,phyC,phyD,and phyE single mutants under 12 different light and temperature regimes,using wild-type(Col-0)seeds as controls.Our results indicate that phyA mutant seeds germinate under red light but are inhibited under far-red light and high temperatures(35℃).phyB mutant seeds germinate at low(15℃)and moderate(25℃)temperatures under both white light and far-red light,but not at high temperatures(35℃).phyC mutant seeds show consistent germination across all conditions except under white light at high temperature(35℃).Both phyD and phyE mutant seeds germinate at low(15℃)and moderate(25℃)temperatures,and under red and white light,but not at high(35℃)temperature,darkness or far-red light.These observations suggest that phyB,phyC,and phyD mutants may have impaired integration of light and temperature cues,whereas phyA and phyE mutants appear to maintain this integrative function.Overall,our findings demonstrate that mutations in phyto-chrome genes can modify seed germination adaptability to varying environmental conditions.

phytochromeArabidopsisseed germinationlight qualitytemperatureecological adaptability

罗燕、刘奇源、吕元兵、吴越、田耀宇、安田、李振华

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贵州大学农学院,贵州省粮油作物分子育种重点实验室,贵州省高等学校功能农业重点实验室,贵阳 550025

光敏色素 拟南芥 种子萌发 光质 温度 生态适应性

国家自然科学基金国家自然科学基金贵州省科技计划贵州省粮油作物分子育种重点实验室项目贵州省高等学校功能农业重点实验室项目

3206051231860420黔科合平台人才[2018]5781号黔科合中引地[2023]008黔教技[2023]007号

2024

10.11983/CBB24008

植物学报
中国科学院植物研究所,中国植物学会

植物学报

CSTPCD北大核心
影响因子：1.254
ISSN：1674-3466
年,卷(期)：2024.59(5)