Tissue Culture and Plant Regeneration of Endangered Plant Davidia involucrate Baill
Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated; the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM + NAA 0. 2 mg · L~(-1) +6-BA 3.0 mg·L~(-1) + AC 2. 0 g·L~(-1); the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM + NAA 0. 05 mg ·L~(-1) + 6-BA 1.0mg·L~(-1)+ GA_3 2. 0 mg· L~(-1) + AC 2.0 g ·L~(-1); the medium for rooting culture was White +IBA3.0 mg·L~(-1) +6-BA 1.0 mg·L~(-1) + AC 2.0 g · L~(-1), under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation.
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