Gene Cloning,Expression and Interaction Protein Analysis of FERONIA in Brassica oleracea var.acephala
FERONIA(FER)receptor kinase plays an important role in pollen and stigma recognition in flowering plants.To analyze the function of receptor-like kinase FERONIA(FER)in the pollination in ornamental kale(Brassica oleracea var.acephala),BoFER gene was cloned from the stigma of the self-incompatibility line(S13-bS13-b)of ornamental kale by RT-PCR,and the cloned cDNA sequence was 2 682 bp,which encoded 893 amino acids containing the highly conserved kinase domain and Ser/Thr kinase binding site,and the expression level of BoFER was analyzed by qRT-PCR.The results showed that the expression of BoFER in stigmas gradually increased after incompatible pollination,but gradually decreased after compatible pollination.Furthermore,the interaction between BoFER and known proteins related to self-incompatibility were analyzed by yeast two-hybrid.The results suggested that BoFER interacted with the kinase domain of S-locus receptor kinase BoSRK13-b.
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