羽衣甘蓝类受体激酶FERONIA基因克隆、表达及与相互作用蛋白分析
Gene Cloning,Expression and Interaction Protein Analysis of FERONIA in Brassica oleracea var.acephala
荀宝茹 1秦洪涛 1马蕊 1郭楠枫 1刘运平 1吴莹 1蓝兴国1
作者信息
- 1. 东北盐碱植被恢复与重建教育部重点实验室,东北林业大学,哈尔滨 150040
- 折叠
摘要
FERONIA(FER)受体激酶在有性生殖中的花粉与柱头信号识别中发挥重要作用,为分析类受体激酶FERONIA(FER)在羽衣甘蓝(Brassica oleracea var.acephala)授粉中的作用,以羽衣甘蓝自交不亲和系(S13-bS13-b)为研究材料,采用RT-PCR从柱头中克隆得到BoFER基因,获得的BoFER cDNA序列,全长2 682 bp编码893个氨基酸,其含有高度保守激酶结构域和Ser/Thr激酶结合位点.利用qRT-PCR技术对BoFER在授粉过程中的表达水平进行分析,结果发现BoFER在不亲和授粉过程其表达量逐渐升高,而在亲和授粉过程中其表达量逐渐降低.利用酵母双杂交分析BoFER与已知的自交不亲和相关蛋白的相互作用,结果显示BoFER与S位点受体激酶BoSRK13-b的激酶域存在相互作用.
Abstract
FERONIA(FER)receptor kinase plays an important role in pollen and stigma recognition in flowering plants.To analyze the function of receptor-like kinase FERONIA(FER)in the pollination in ornamental kale(Brassica oleracea var.acephala),BoFER gene was cloned from the stigma of the self-incompatibility line(S13-bS13-b)of ornamental kale by RT-PCR,and the cloned cDNA sequence was 2 682 bp,which encoded 893 amino acids containing the highly conserved kinase domain and Ser/Thr kinase binding site,and the expression level of BoFER was analyzed by qRT-PCR.The results showed that the expression of BoFER in stigmas gradually increased after incompatible pollination,but gradually decreased after compatible pollination.Furthermore,the interaction between BoFER and known proteins related to self-incompatibility were analyzed by yeast two-hybrid.The results suggested that BoFER interacted with the kinase domain of S-locus receptor kinase BoSRK13-b.
关键词
羽衣甘蓝/FERONIA/自交不亲和性/表达分析/酵母双杂交Key words
Brassica oleracea var.acephala/FERONIA/self-incompatibility/expression analysis/yeast two-hybrid引用本文复制引用
基金项目
黑龙江省大学生创新创业训练计划项目(S202310225227)
国家自然科学基金面上项目(31870300)
出版年
2024
国家科技期刊平台
NSTL
万方数据