Anatomical Structure and Cell Wall Components Changes in Chestnut'Yanshan Hongli'Female Flowers at Developmental Stages
To investigate the anatomical characteristics of female flowers and the changes of cell wall components of Castanea mollissima during the growth and development,'Yanshan Hongli'was used as materials,and the internal morphological changes were observed by paraffin sections and the changes of cell wall components were observed by fluorescence staining.According to its internal microstructure changes,the collected samples were determined at the four most important development stages:flower primordium differentiation stage,stigma primordium differentiation stage,stigma elongation stage and flowering stage.The results showed that the fluorescence intensity of cellulose in the cell wall of female flowers gradually increased during the development.Especially during the process of stigma elongation,the fluorescence intensity of cellulose in stigma and ovary was significantly enhanced.In addition,the fluorescence intensity of esterified pectin was always higher than that of acidic pectin during the four development stages and the fluorescence intensity of pectin in stigma and ovary was stronger than that of other parts.These results revealed the changes of cell wall components during the growth and development of chestnut female flowers,and provided important clues for further understanding the mechanism of chestnut growth and development.
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