Meristematic Nodule Induction and Adventitious Shoot Differentiation in Distant Hybrid Tree Peony
To explore a simple and suitable method for inducing meristematic nodules in multiple cultivars,the petioles of inter-subsectional and inter-sectional distant hybrid tree peony were used as explants,and the conditions for the differentiation of meristematic nodules into adventitious shoot in some cultivars were also identified.The results showed that meristematic nodules were induced by one step in 10 cultivars with 52.08%-83.33%induction rates,the thin layers of petioles of 11 cultivars were continuously cultured on SH medium containing 0.5 mg·L-1 NAA+1.0 mg·L-1 CPPU+0.5(or 0)mg·L-1 TDZ for 60-100 d in the dark,and meristematic nodules induced by one step were generated indirectly from the callus.Adventitious shoot differentiation rate of three cultivars was 6.67%-33.33%when meristem nodules were added to the modified WPM medium containing 0.5(or 1)mg·L-1 CPPU+0.5 mg·L-1 TDZ under light condition and sub-cultured every 15 days.Lower concentrations of CPPU and TDZ together promoted adventitious shoot differentiation,but high concentrations of CPPU alone were not conductive to adventitious shoot differentiation.The general medium for one-step induction of meristematic nodules was screened,and adventitious shoot differentiation was achieved in some cultivars,which provided an important reference for the establishment of plant regeneration system for distant hybrid tree peonies.
Paeonia×lemoineiintersectional hybrids of Paeoniameristematic noduleadventitious shoot
万方数据
维普
