The Bioinformatic Analysis on the Plant Isopentenyl Pyrophosphate Isomerase
Isopentenyl pyrophosphate isomerase(IPP)is responsible for the reversible isomerization of isopentenyl pyrophosphate and dimethylallylpyrophosphate(DMAPP),playing an important role in the biosynthesis of downstream terpenoid products.In this paper,31 IPP genes were identified from genomes of various mono-and dicotyledonous plants such as Oryza sativa and Arabidopsis thaliana by bioinformatics method.Phylogenetic analysis indicated that these IPPs were divided into three clades,in which those from monocotyledon plants formed a monophyletic clade.IPPs within the same clade shared similar domains and might have similar biological functions.The analysis on cis-regulatory elements of O.sativa and A.thaliana IPP genes exhibited multiple response elements such as drought response elements.Finally,quantitative PCR results showed that the expression patterns of IPP transcription levels in O.sativa and A.thaliana were diversified.These above results provided theoretical basis for systematic understanding and further application of IPPs.