Cloning and Drought Tolerance Functional Validation of PvAQP Gene in Psammochloa villosa (Poaceae)
analysis showed that PvAQP protein was localized in the nucleus. (6)Among different species,the homology with amino acid sequences of PvAQP gene was 95.34％. P. villosa had the closest genetic relationship with Stipa caucasica. (7)The expression of PvAQP gene was tissue-specific,with significant differences in the roots,stems,and leaves of P. villosa. (8)With the increase of simulated drought-stress time,the activities of superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT),and the content of proline(PRO) first increased and then decreased in transgenic tobacco and wild type tobacco. Compared with wild type tobacco,the express levels in the transgenic tobacco were higher,while the content of malondialdehyde(MDA) showed an increasing trend,and the content of MDA in transgenic PvAQP gene-type was significantly lower than that of wild type. The results suggested that PvAQP gene might be involved in the physiological process of drought tolerance within P. villosa,and provided the theoretical evidence for revealing the molecular mechanism of PvAQP gene responding to drought tolerance in the future.
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