Effects of Bacillus subtilis on the Growth and Bulb Metabolism of Lilium davidii var. unicolor
In order to investigate the effect of Bacillus subtilis on the growth and development and its bulb metabolites of Lilium davidii var. unicolor,L. davidii var. unicolor was used as the experimental material,CK (peat+perlite) and TR(peat+perlite+B. subtilis) treatments were conducted using B. subtilis by root irrigation. The growth changes of L. davidii var. unicolor at different stages (budding period,flowering period and semi-dry period) were observed,the differential metabolites in bulbs in semi-dry period were detected and analyzed by LC-MS method. The results showed that compared with CK,TR treatment had promoting effects on plant height,stem diameter,number of blades,chlorophyll content,basal root number,stem root number and circumference of L. davidii var. unicolor,respectively. Compared with CK,the plant height and stem diameter under TR treatment showed significant differences(P<0.05) at the budding period,which increased by 20.06％ and 40.68％,respectively. The number of leaves increased significantly at budding period and flowering period (P<0.05),reaching 1.35 times and 1.45 times of CK,respectively. At the flowering period,compared with CK,the circumference of bulbs and the number of rooting of L. davidii var. unicolor. stems treated with TR were increased by 15.87％ and 58.14％,respectively,and the difference was significant(P<0.05). In the bulb metabolites analysis,408 differential metabolites were screened from the bulbs in CK vs TR groups. A total of 14 metabolic pathways were enriched,and the differential metabolites were mainly enriched in amino acid metabolism and synthesis pathways. In summary,the application of B. subtilis could promote the growth and development of L. davidii var. unicolor and affect the metabolism of nutrients in the bulbs.
Lilium davidii var. unicolorBacillus subtilisagronomic traitsdifferential metabolite
万方数据
维普
