Effect of Fe Element on Bulbil Formation and Tube Seedling Growth of Pinellia ternata
To obtain an optimal Fe2+concentration for culturing test tube bulbils of Pinellia ternata,the concentrations of Fe2+in 1/2MS basic medium were changed in gradient;the formation time,induction rate and the above-ground and underground morphological indices of test-tube plantlets were investigated. The number and distribution of starch granules and the content of chlorophyll in leaves and the content of effective components in test tube bulbils were assayed. The results showed that the optimal Fe2+concentration in 1/2MS basic medium for culturing test tube bulbils was 9.2×10-5 mol·L-1. In addition,the complete bulbils could not be formed under Fe deficiency. This indicated that Fe was an essential trace element in growth of P. ternata. However,the bulbils formed under high concentration of Fe2+were very small and failed to germinate normally.
Pinellia ternata(Thunb.)Breit.test tube bulbilstube seedlingsstarch grainFe element
万方数据
维普
