Methods for Reducing the Vitrification Rate and Promoting Rooting Formation of Dianthus plumarius
The shoot tips of Dianthus plumarius were used as explants,and the optimal disinfection duration with HgCl₂ and the suitable proliferation medium were screened out. The effects of adding activated carbon (AC),chlormequat(CCC),polyvinylpyrrolidone(PVP),and paclobutrazol(PP333) on the vitrification aseptic seedlings of D. plumarius,as well as the impact of different plant hormone ratios on rooting were investigated. The results indicated that the appropriate disinfection method for shoot tips of D. plumarius was 75％ ethanol for 30 s followed by 0.1％ HgCl2 for 10 min,and the survival rate was 86.63％. The suitable proliferation medium was identified as MS supplemented with 2 mg·L-16-benzylaminopurine(6-BA),0.05 mg·L-1 Naphthaleneacetic acid (NAA),30 g·L-1 sucrose,and 7 g·L-1 agar,resulting in cluster buds proliferation rate of 90.28％,with an average bud height of 5.25 cm and a high proliferation coefficient of 12.31. In reducing the vitrification effect aseptic seedlings of D. plumarius,the effect from high to low was CCC,AC,PP333 and PVP. Adding 3 mg·L-1 CCC in 1/2 MS medium was beneficial for overcoming the vitrification phenomenon and promoting plant growth. Adding 0.5 mg·L⁻¹ NAA to the medium formula that is optimal for overcoming the vitrification rate is appropriate for the rooting of sterile seedlings of D. plumarius semperflorens. The rooting rate can reach 100％,with an average root length of 16.83 cm and an average number of 15.66 roots per individual plant. The findings provided theoretical and technical supports for the rapid propagation and industrial seedling production of D. plumarius.
万方数据
维普
