Study on the Mechanism of Sinomenine in the Treatment of Knee Osteoarthritis Based on RNA-Seq Technique
Objective To observe the effect of sinomenine on the transcriptome of articular cartilage in rats with knee osteoarthritis(KOA)induced by sodium iodoacetate(MIA).Methods Totally 30 SD rats were randomly divided into three groups:blank group(CK),model group(M)and treatment group(T),10 in each group.The osteoarthritis model was established by intra-articular injection of sodium iodoacetate(2 mg/50 íL normal saline)into the right lower limb of the M group and T group.The knee joint diameter of the right lower limb of all rats was measured every week after injection.After 21 days of modeling,rats in the T group began to be injected with sinomenine(60 mg/kg)once a day for 10 consecutive days.Eight h after the last administration,the right lower limb joints were cut open after anesthesia.Part of the joints were removed and fixed and decalcified for histopathological analysis.Part of the articular cartilage was removed and the total RNA was extracted and sequenced.The gene expressions in the cartilage tissues of the three groups were detected,and the sequencing data were analyzed,including differential expression analysis,gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results Sinomenine reduced the diameter of knee joint,attenuated the injury of articular cartilage and lowered Mankin's score(P＜0.05).There were 24 genes with significant differences among the three groups of experimental rats,which were significantly enriched in monocyte chemotaxis,CCR chemokine receptor binding,KEGG-enriched cytokine receptor interaction and chemokine signal pathway in the functional classification of GO.Of them,the main target groups were C-C motif chemokine ligand(CCL)4,CCL9 and Prkag3.Conclusions Sinomenine exerted its therapeutic effect on osteoarthritis through multiple pathways and multiple targets.The key targets CCL4,CCL9,Prkag3 and their related signal pathways may be the main mechanism of sinomenine in the treatment of osteoarthritis.
国家科技期刊平台
NSTL
万方数据
维普
