摘要
目的 观察半夏厚朴汤(BHF)及其拆方对慢性间歇性低氧(CIH)小鼠机体铁代谢的调节作用.方法 42只雄性C57BL/6N小鼠随机分为常氧对照组(Con)、CIH模型组(CIH)、全方组(CIH+BHF)、苏叶组(CIH+苏叶)、厚朴组(CIH+厚朴)、行气组(CIH+厚朴、苏叶)、化痰饮组(CIH+半夏、茯苓、生姜),每组6只.连续CIH暴露和灌胃给药处理21 d后检测相关指标.比色法检测血清铁,HE染色观察肝脏及十二指肠形态结构、Perls'染色观察肝脏、脾脏、十二指肠铁含量,Western Blot及免疫组织化学染色检测肝脏铁调素(hepcidin)、转铁蛋白受体1(transferrin receptor 1,TfR1)、铁泵蛋白 1(ferroportin 1,FPN1)、铁蛋白轻链(ferritin light chain,FTL)蛋白表达.结果 与 Con 组比较,CIH组小鼠血清铁含量降低,小肠黏膜(十二指肠)与肝脏组织结构损伤,肝脏、小肠铁沉积增加(P＜0.05),脾脏铁含量减少(P＜0.05),肝脏TfR1、FTL、hepcidin蛋白表达升高(P＜0.05),FPN1蛋白表达下降(P＜0.05).与CIH组比较,全方组、行气组小鼠血清铁水平上调(P＜0.05),全方组、苏叶组、厚朴组、行气组小肠黏膜与肝脏组织形态较为完整,肝脏、小肠铁沉积减轻(P＜0.05),全方组、行气组脾脏铁含量回升(P＜0.01,P＜0.05),全方及行气组肝脏hepcidin、TfR1、FPN1和FTL蛋白表达紊乱改善(P＜0.05).结论 半夏厚朴汤及其拆方能够不同程度改善CIH暴露引起的铁代谢紊乱,其中行气药可能发挥重要作用.
Abstract
Objective To observe the effects of Banxia Houpo Formula(BHF)and its disassembled prescriptions on iron metabolism in mice with chronic intermittent hypoxia(CIH).Methods Forty-two male C57BL/6N mice were randomly divided into 7 groups:control group(Con),CIH model group(CIH),BHF group(CIH+BHF),Suye group(CIH+perilla leaf),Houpo group(CIH+Mangnolia officinalis),Moving qi group(CIH+perilla leaf+Mangnolia officinalis),and Resolving phlegm and fluid retention group(CIH+Pinellia ternate,Poria cocos,and fresh ginger),6 mice in each group.After 21 days of CIH exposure and intragastric administration,the indicators were detected.Serum iron was measured by colorimetric method,morphological structure of liver and duodenum was observed by hematoxylin-eosin staining,iron content and distribution in liver,spleen,and duodenum were observed by Perls'staining,liver transferrin receptor 1(TfR1),ferroportin 1(FPN1),ferritin light chain(FTL)and hepcidin proteins were detected by Western Blot and immunohistochemical staining.Results Compared with the Con group,the CIH group's serum iron level was lower,and the duodenal mucosa and liver tissue structure were damaged,iron deposition in the liver and small intestine increased(P＜0.05),the spleen's iron content reduced(P＜0.05).There was an increase in hepatic TfR1,FTL,hepcidin protein expression(P＜0.05),and a decrease in FPN1 protein expression(P＜0.05)in CIH group.Compared with the CIH group,the morphology of the small intestinal mucosa and liver tissue was relatively intact,and the iron deposition in the liver and intestine was reduced in the BHF,Suye,Houpo,and Moving qi groups(P＜0.05),while the spleen iron content increased in the BHF and Moving qi groups(P＜0.01,P＜0.05).In the BHF and Moving qi groups,the disordered expression of liver hepcidin,TfR1,FPN1,and FTL alleviated(P＜0.05).Conclusion BHF and its disassembled prescriptions can alleviate the iron metabolism disorder caused by CIH exposure to varying degrees,and moving qi herbs may be particularly beneficial in this regard.
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