Banxia Houpo Formula and Its Disassembled Prescriptions Improves Iron Metabolism in Mice with Chronic Intermittent Hypoxia
Objective To observe the effects of Banxia Houpo Formula(BHF)and its disassembled prescriptions on iron metabolism in mice with chronic intermittent hypoxia(CIH).Methods Forty-two male C57BL/6N mice were randomly divided into 7 groups:control group(Con),CIH model group(CIH),BHF group(CIH+BHF),Suye group(CIH+perilla leaf),Houpo group(CIH+Mangnolia officinalis),Moving qi group(CIH+perilla leaf+Mangnolia officinalis),and Resolving phlegm and fluid retention group(CIH+Pinellia ternate,Poria cocos,and fresh ginger),6 mice in each group.After 21 days of CIH exposure and intragastric administration,the indicators were detected.Serum iron was measured by colorimetric method,morphological structure of liver and duodenum was observed by hematoxylin-eosin staining,iron content and distribution in liver,spleen,and duodenum were observed by Perls'staining,liver transferrin receptor 1(TfR1),ferroportin 1(FPN1),ferritin light chain(FTL)and hepcidin proteins were detected by Western Blot and immunohistochemical staining.Results Compared with the Con group,the CIH group's serum iron level was lower,and the duodenal mucosa and liver tissue structure were damaged,iron deposition in the liver and small intestine increased(P＜0.05),the spleen's iron content reduced(P＜0.05).There was an increase in hepatic TfR1,FTL,hepcidin protein expression(P＜0.05),and a decrease in FPN1 protein expression(P＜0.05)in CIH group.Compared with the CIH group,the morphology of the small intestinal mucosa and liver tissue was relatively intact,and the iron deposition in the liver and intestine was reduced in the BHF,Suye,Houpo,and Moving qi groups(P＜0.05),while the spleen iron content increased in the BHF and Moving qi groups(P＜0.01,P＜0.05).In the BHF and Moving qi groups,the disordered expression of liver hepcidin,TfR1,FPN1,and FTL alleviated(P＜0.05).Conclusion BHF and its disassembled prescriptions can alleviate the iron metabolism disorder caused by CIH exposure to varying degrees,and moving qi herbs may be particularly beneficial in this regard.
Banxia Houpo Formulairon metabolismchronic intermittent hypoxiaperilla leafcortex magnoliae officinalispromoting the circulation of qitreating qi before treating phlegmcompatibilityChinese herbal compound
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