Effect and Mechanism of Liuwei Buqi Formula on Pyroptosis in Chronic Obstructive Pulmonary Disease
Objective To investigate the effect of Liuwei Buqi Formula on pyroptosis in chronic obstructive pulmonary disease(COPD).Methods According to the random number table method,the rats were divided into normal control group,model group,Guilong Kechuanning group and low,medium,high dose group of Liuwei Buqi Formula,with 10 rats in each group.The experimental rats models of COPD were established by smoked and lipopolysaccharide(LPS)tracheal drip.After intervention,the pulmonary function,pathological changes in bronchopulmonary tissues and pyroptosis situation of rats were detected.Apart from that,the NOD-like receptor thermal protein domain associated protein 3(NLRP3)and GSDMD-N alterations in lung tissue,levels of inflammatory factors interleukin-18(IL-18)and interleukin-1 β(IL-1 β)in broncho alveolar lavage fluid(BALF),lung tissue reactive oxygen species(ROS)level,lung tissue protein expression and mRNA levels of NLRP3,cysteinyl aspartate specific proteinase-1(Caspase-1),GSDMD,and apoptosis-associated speck-like protein containing a CARD(ASC)were detected.Results Compared with normal control group,the model group of rats showed lung function change,lung tissue damage,cell membrane rupture,increased of IL-18,IL-1 β,ROS(P＜0.01),and expression of NLRP3,Caspase-1,GSDMD,ASC protein and mRNA significantly increased in lung tissue(P＜0.01,P＜0.05).Compared with the model group,lung function in rats improved,lung tissue damage was alleviated,the pyroptosis was reduced,levels of IL-18,IL-1 β,ROS decreased(P＜0.01),the protein and mRNA expression of NLRP3,Caspase-1,GSDMD,GSDMD-N,ASC decreased in each drug group(P＜0.01).The most significant changes which in Liuwei Buqi Formula high-dose group.Conclusion Liuwei Buqi Formula could reduce IL-18,IL-1 β,ROS levels in COPD rats,decrease airway inflammation and oxidative stress,inhibit lung tissue damage and pyroptosis,and improve lung function that the mechanism may be achieved by regulating the ROS/NLRP3/Caspase-1 signaling pathway.
chronic obstructive pulmonary diseaseinflammatory reactionpyroptosisreactive oxygen species(ROS)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)/cysteinyl aspartate specific proteinase-1(Caspase-1)signaling pathwayChinese herbal co
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