Mechanism of Modified Xuanbai Chengqi Decoction Combined with Western Medicine in Improving Pneumonia in Influenza Virus/Streptococcus Pneumoniae Co-infected Mice
Objective To explore the pathogenesis of co-infection with influenza virus/Streptococcus pneumoniae(Ⅳ/Spn)in mice and the therapeutic effects of Chinese medicine formula Modified Xuanbai Chengqi Decoction using the tandem mass tag(TMT)labelled quantitative proteomics technology.Methods Forty young healthy male SPF grade Balb/c mice were divided into normal group,model group,Chinese medicine group,Western medicine group,and integrated Chinese and Western medicine group,8 mice in each group.After successful modelling,the mice were treated with Modified Xuanbai Chengqi Decoction(0.15 g/mL),oseltamivir(0.25 mg/mL)/ceftriaxone(0.48 g/mL),and combined Chinese and Western medicine intervention,respectively,while the normal group and model group were given equal volumes of normal saline by gavage.Lung tissue samples were collected after 7 days,and the samples from each group of mice were isolated.All samples were labelled and quantified using reagents TMT 10,followed by protein digestion,TMT peptide labelling and peptide fractionation,and LC-MS/MS analysis.Differential proteins were identified using a database search method and subjected to bioinformatics analysis.Results Compared with normal group,the lung index of mice in model group increased(P＜0.05).Compared with the model group,the lung index of mice in all administration groups decreased(P＜0.05,P＜0.01),and the inhibition rate of lung index in Chinese medicine group,Western medicine group and integrated Chinese and Western combination group was 68.66％,77.61％and 85.07％,respectively.Compared with the normal group of mice,co-infection with Ⅳ/Spn pneumonia in mice led to changes in the expression of 259 proteins,involving complement and coagulation cascades,amino sugar and nucleotide sugar metabolism,Fcγ-mediated phagocytosis,and other signalling pathways.After intervention with Modified Xuanbai Chengqi Decoction,changes in the expression of 13 proteins were observed,mainly enriched in pathways such as propionate,butyrate,and galactose metabolism.Intervention with Western medicine resulted in changes in the expression of 97 proteins,mainly enriched in pathways such as complement and coagulation cascades,amino sugar and nucleotide sugar metabolism,and retinoic acid-inducible gene 1(RIG-1)-like receptor signalling.After intervention with integrated Chinese and Western medicine,changes in the expression of 144 differential proteins were observed,mainly enriched in pathways such as extracellular matrix(ECM)interaction,small cell lung cancer,and retinoic acid-inducible gene 1 like receptor signalling.Conclusions The immune-inflammatory reaction occurring in the lung tissue of mice is the main cause of Ⅳ/Spn co-infection pneumonia.Modified Xuanbai Chengqi Decoction can correct Ⅳ/Spn co-infection pneumonia by regulating intestinal dysbiosis and substance energy metabolism.Western medicine can alleviate lung inflammation after Ⅳ/Spn co-infection by immune regulation.Integrated Chinese and Western medicine can regulate the body's immune and inflammatory responses while providing substance energy for the body.
Modified Xuanbai Chengqi DecoctionⅣ/Spn co-infectionTMT labeling quantitative proteomics techniquesbioinformaticsintegrative medicine
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