Mechanism of Qilan Decoction's Effect on the Proliferation and Apoptosis of Prostate Cancer DU145 Cells Based on the miR-1297/PTEN/PI3K/AKT Signaling Pathway
Objective To explore the possible mechanism of Qilan Decoction's effect on the proliferation and apoptosis of prostate cancer cells.Methods DU145 cells were divided into negative control group(NC mimics),Qilan Decoction group(NC mimics+QLF),miR-1297 over-expression group(miR-1297 mimics),miR-1297 over-expression+Qilan Decoction group(miR-1297 mimics+QLF),AKT activator group(SC79),AKT activator+Qilan Decoction group(SC79+QLF).CCK-8 method and EdU experiment were used to detect cell proliferation in each group.Flow cytometry were used to detect cell cycle and apoptosis in each group.RT-PCR was used to detect the mRNA expression of miR-1297,phosphatase and tensin homologue deleted on chromosome 10(PTEN),and protein kinase B(AKT).Western Blot was used to detect the expression of PTEN,phosphatidylinositol-3 kinase(PI3K),p-AKT,Cyclin D1,B-cell lymphoma/leukemia 2(Bcl-2),Bcl-2 related X protein(Bax),and Cleaved-caspase 3.Results Compared with negative control group,the activity,proliferation rate,EdU positive cell rate,G1 phase cell proportion,miRNA 1297 mRNA protein expression,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression of DU145 cells in the Qilan Decoction group decreased,while the G2 phase cell proportion,Q1-LR(early apoptosis)+Q1-UR(late apoptosis),PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).The miR-1297 over-expression group and AKT activator group showed an increase in DU145 cell activity,proliferation rate,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression,while the expression of Q1-LR+Q1-UR,PTEN proteins decreased(P＜0.01).Compared with the miR-1297 over-expression group,miR-1297 over-expression group+Qilan Decoction showed a decrease in DU145 cell activity,proliferation rate,G1 phase cell proportion,miRNA 1297 mRNA expression,PI3K,p-AKT,Cyclin D1,and Bcl-2 proteins,while the G2 phase cell proportion,Q1-LR+Q1-UR,PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).Compared with the AKT activator group,AKT activator+Qilan Decoction group showed a decrease in DU145 cell activity,proliferation rate,EdU positive cell rate,S phase cell proportion,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression,while the G1 and G2 cell proportion,Q1-LR+Q1-UR,PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).Conclusion Qilan Decoction could inhibit the proliferation of DU 145 cells and promote the apoptosis of DU 145 cells by regulating the miR-1297/PTEN/PI3K/AKT signaling pathway.
国家科技期刊平台
NSTL
万方数据
