Daotan Xixin Decoction Inhibits Alzheimer's Disease Oligodendrocyte Apoptosis by Regulating AMPK/SIRT1/NF-κB Signaling Pathway Protein Expression
Objective To investigate the effect of Daotan Xixin Decoction on MO3.13 human oligodendrocytic cell apoptosis in an Alzheimer's disease(AD)cell model,and to explore its regulatory mechanism.Methods In vitro model of Alzheimer's disease was established using MO3.13 cells induced by amyloid-β142(Aβ1-42).The cells were divided into the following groups:blank control group,AD cell model group,Daotan Xixin Decoction containing serum groups(low,medium and high concentration),and Donepezil containing serum group,with 3 samples in each group.Cell activity was detected using CCK8,apoptosis rate was detected by flow cytometry,and the content of adenosine triphosphate(ATP)in the cell supernatant was detected using an ATP content detection kit.Western Blot was used to detect the expression abundance of phosphorylated AMP-activated protein(p-AMPK),silent information regulator 1(SIRT1),B-cell lymphoma-2(Bcl-2),nuclear factor kappa B(NF-κB)and Caspase3 proteins.Results Compared with the blank control group,the cell activity in the AD cell model group decreased(P＜0.001),the apoptosis rate increased(P＜0.001),the ATP content decreased(P＜0.001),the protein expression of p-AMPK,SIRT1,and Bcl-2 decreased(P＜0.001),and the protein expression of NF-κB and Caspase3 increased(P＜0.001).Compared with the AD cell model group,the cell activity of the high concentration Daotan Xixin Decoction group and Donepezil group improved(P＜0.01),the apoptosis rate decreased(P＜0.001),the ATP content increased(P＜0.001),the protein expressions of p-AMPK,SIRT1,and Bcl-2 increased(P＜0.001),and the protein expressions of NF-κB and Caspase3 decreased(P＜0.001).Conclusion Daotan Xixin Decoction improves ATP content in AD oligodendrocytes by activating the AMPK/SIRT1/NF-κB signaling pathway and inhibits the inflammatory and pro-apoptotic effects of A β 1-42 on AD oligodendrocytes.
Daotan Xixin DecoctionAlzheimer's diseaseAMPK/SIRT1/NF-κB signaling pathwayoligodendrocyteapoptosisintegrative medicine
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