Mechanism of Dendrobium officinale from Longhu Mountain in Jiangxi Province on Wei Yin Deficiency Mice
Objective Through cyclic adenosine monophosphate(cAMP)/protein kinase A(PKA)/aquaporins(AQPs)pathway and gastrointestinal tract function,to investage the mechanism of Wei yin deficiency mice model based on the different extracting processes(water extract,alcohol extract and polysaccharide)of Dendrobium officinale from Longhu mountain in Jiangxi Province.Methods Totally120 male ICR mice were randomly divided into normal group,model group,Yiwei Decoction group(15 g·kg-1),Omeprazole group(1.7 mg·kg-1),and also every different extracting processes including high dose of Dendrobium officinale group(4.2 g·kg-1),low dose of Dendrobium officinale group(2.1 g·kg-1),12 mice in each group.The Wei yin deficiency mice model was established by intragastric administration with a mixture of liquor and pepper liquid.The drug intervention groups received intervention starting from the 30th day of modeling and continued until the 70th day.The body weight,water and diet intake were measured every 3 days during the experiment.The submandibular gland index was calculated,and the fecal water content and saliva secretion were measured after the experiment.The gastric tissue was observed by HE staining.The contents of vasoactive intestinal peptide(VIP)and cyclic adenosine monophosphate(cAMP)in serum were determined by ELISA.The protein expressions of PKA,phosphorylated PKA,AQP3,AQP4,AQP5,Zonula occluden-1(ZO-1),and E-cadherin were determined by Western Blot.Results Compared with the normal group,the food intake,water intake,body weight,and the content of saliva secretion and fecal water decreased in the model group(P＜0.05).HE staining showed that the gastric mucosal tissue became thinner and more defective,leading to the loss of the muscle layer and disordered,arrangement of glands,thus finally resulted in inflammatory cell infiltration.The content of VIP and cAMP in serum decreased(P＜0.05),the expressions of p-PKA/PKA,AQP3,AQP4,and ZO-1 protein were down-regulated(P＜0.05,P＜0.01),the expressions of AQP5 and E-cadherin were up-regulated(P＜0.05,P＜0.01).Compared with the model group,administration of different Dendrobium officinale extracts resulted in an increase in saliva secretion in high doses of water and polysaccharide groups(P＜0.05),and the content of fecal water increased in the Omeprazole group,alcohol and polysaccharide groups(P＜0.05,P＜0.01).Omeprazole group and all of these three extracts,excepting for the lowdose of alcohol extract,the content of VIP and cAMP increased(P＜0.05),the cAMP content increased in the Yiwei Decoction group(P＜0.05).Excepting for the high dose of alcohol extract group,the p-PKA/PKA ratio were up-regulated(P＜0.05).Except Yiwei Decoction group,the expressions of ZO-1 protein were up-regulated in each group(P＜0.05),the expression of E-cadherin protein were down-regulated in three extracts groups(P＜0.05,P＜0.01).The expressions of AQP3 protein were up-regulated in the Omeprazole group,and high dose group of aqueous extract and polysaccharide(P＜0.05,P＜0.01),the expressions of AQP4 protein were up-regulated in the high dose group of three extracts(P＜0.01),the expressions of AQP5 protein were down-regulated in the high and low doses group of polysaccharide(P＜0.01,P＜0.05).Conclusions Dendrobium officinale from Longhu mountain in Jiangxi Province could improve the symptoms of mice model with Wei yin deficiency,and its mechanism is related to cAMP/PKA/AQPs pathway and tight junctions.Among them,the high dose of water extract and polysaccharide has an advantage in promoting salivary secretion and AQP3,AQP4 expression,polysaccharide has an advantage in down-regulating AQP5 expression.
Dendrobium officinaleDendrobium officinale polysaccharidesWei yin deficiencyaquaporinzonula occludes proteinChinese herbal medicine
国家科技期刊平台
NSTL
万方数据
