Yiyuan Moxibustion Improves Urodynamics of Neurogenic Bladder Rats after Spinal Cord Injury Based on Regulating TRPV1/C-kit/HCN1
Objective To observe the effect of Yiyuan moxibustion(YM)on urodynamics of neurogenic bladder(NB)rats after sacral spinal cord injury(SCI),and to explore its mechanism based on transient receptor potential vanillic acid 1(TRPV1),C-kit and hyperpolarization-activated cyclic nucleotide cation channel 1(HCN1)pathway.Methods Totally 72 SD rats were randomly divided into sham operation group(n=12),model group(n=15),YM group(n=15),inhibitor group(n=15)and YM+inhibitor group(n=15).The rat model of NB after SCI was established by modified Hassan Shaker spinal cord transection method.The sham operation group only exposed the spinal cord and did not transect.Except sham operation group,each group was given daily manual urination.The YM group was treated with YM at Shenque(CV8)and Guanyuan(CV4).The inhibitor group was given TRPV1 inhibitor capsazepine(2.5 mL,100 nmol/L,30 min)for bladder perfusion.The YM+inhibitor group was given YM and capsazepine intervention,the intervention course for all was 14 consecutive days.The bladder function of rats was detected by urodynamics,the morphological changes of bladder tissue were observed by HE staining,and the ATP content in bladder tissue was detected by colorimetry.The positive expression of interstitial cells of Cajal(ICC)and HCN1 in bladder tissue were observed by immunofluorescence double staining.The protein and mRNA expressions of TRPV1,C-Kit and HCN1 in bladder tissues were detected by Western Blot and RT-PCR,respectively.Results The bladder tissue structure of rats in the sham operation group was complete and orderly.The bladder epithelial cells in the model group and inhibitor group were disordered,the lamina propria was damaged,the muscle bundles were blurred,the fibers were contracted,the tissue edema and hemorrhage were observed.YM group and the YM+inhibitor group were more regular in the arrangement of bladder tissue structure,the vacuolar cells were decreased,the muscle fibers were integrity,and the tissue edema and bleeding were relief compared with model group and inhibitor group,respectively.Compared with the sham operation group,the maximum bladder capacity and bladder compliance increased(P＜0.01),and the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence intensity,TRPV1,C-Kit,HCN1 protein and mRNA expression decreased in model group(P＜0.01).Compared with the model group and inhibitor group,the maximum bladder capacity and bladder compliance decreased(P＜0.01),and the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence intensity,TRPV1,C-Kit,HCN1 protein and mRNA expression increased in YM group and YM+inhibitor group(P＜0.05,P＜0.01),respectively.The maximum bladder capacity and bladder compliance increased(P＜0.01),the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence,TRPV1,C-Kit,HCN1 protein and mRNA expression decreased in inhibitor group than model group(P＜0.05,P＜0.01).Conclusions YM can improve the urodynamics of NB rats after SCI,and its mechanism may related to the regulation of TRPV1/C-ki/HCN1 pathway in bladder tissue.
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