Acupuncture ameliorates atopic dermatitis by modulating gut barrier function in a gut microbiota-dependent manner in mice

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外文标题：Acupuncture ameliorates atopic dermatitis by modulating gut barrier function in a gut microbiota-dependent manner in mice

外文摘要：Objective:Atopic dermatitis(AD)is a chronic inflammatory skin disease that may be linked to changes in the gut microbiome.Acupuncture has been proven to be effective in reducing AD symptoms without seri-ous adverse events,but its underlying mechanism is not completely understood.The purpose of this study was to investigate whether the potential effect of acupuncture on AD is gut microbiota-dependent.Methods:AD-like skin lesions were induced by applying MC903 topically to the cheek of the mouse.Acupuncture was done at the Gok-Ji(LI11)acupoints.AD-like symptoms were assessed by lesion scores,scratching behavior,and histopathological changes;intestinal barrier function was measured by fecal output,serum lipopolysaccharide levels,histopathological changes,and mRNA expression of markers involved in intestinal permeability and inflammation.Gut microbiota was profiled using 16S rRNA gene sequencing from fecal samples.Results:Acupuncture effectively improved chronic itch as well as the AD-like skin lesions with epidermal thickening,and also significantly altered gut microbiota structure as revealed by β-diversity indices and analysis of similarities.These beneficial effects were eliminated by antibiotic depletion of gut microbiota,but were reproduced in gut microbiota-depleted mice that received a fecal microbiota transplant from acupuncture-treated mice.Interestingly,AD mice had intestinal barrier dysfunction as indicated by increased intestinal permeability,atrophy of the mucosal structure(reduced villus height and crypt depth),decreased expression of tight junctions and mucus synthesis genes,and increased expression of inflammatory mediators in the ileum.Acupuncture attenuated these abnormalities,which was gut microbiota-dependent.Conclusion:Acupuncture ameliorates AD-like phenotypes in a gut microbiota-dependent manner and some of these positive benefits are explained by modulation of the intestinal barrier,providing new per-spective for non-pharmacological strategies for modulating gut microbiota to prevent and treat AD.

外文关键词：

Atopic dermatitisAcupunctureGut microbiotaIntestinal barrier function

作者：

Mijung Yeom、Sora Ahn、Dae-Hyun Hahm、Sun-Young Jang、Se Hoon Jang、Su-Yang Park、Jae-Hwan Jang、Jihan Park、Ju-Young Oh、In-Seon Lee、Kyuseok Kim、Soon-Kyeong Kwon、Hi-Joon Park

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作者单位：

Acupuncture and Meridian Science Research Center,College of Korean Medicine,Kyung Hee University,Seoul 02447,Republic of Korea

Department of Physiology,School of Medicine,Kyung Hee University,Seoul 02447,Republic of Korea

BioNanocomposite Research Center,Kyung Hee University,Seoul 02447,Republic of Korea

Department of Science in Korean Medicine,College of Korean Medicine,Graduate School,Kyung Hee University,Seoul 02447,Republic of Korea

College of Korean Medicine,Kyung Hee University,Seoul 02447,Republic of Korea

Department of Ophthalmology,Otorhinolaryngology and Dermatology of Korean Medicine,College of Korean Medicine,Kyung Hee University,Seoul 02447,Republic of Korea

Division of Applied Life Science(BK21),Gyeongsang National University,Jinju 52828,Republic of Korea

Department of Anatomy & Information Sciences,College of Korean Medicine,Kyung Hee University,Seoul 02447,Republic of Korea

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关键词：

Atopic dermatitis Acupuncture Gut microbiota Intestinal barrier function

出版年：

2024

DOI：

10.1016/j.joim.2024.07.004

结合医学学报(英文版)

上海市中西医结合学会,上海长海医院

结合医学学报(英文版)

CSTPCD

影响因子：0.711

ISSN：2095-4964

年,卷(期)：2024.22(5)