Effects of Icariin on Tumor Growth and Cell Cycle in Prostate Cancer Orthotopic Transplantation Tumor Mice
Objective To investigate the effects of icariin on tumor growth,cell cycle and expression of phos-phatase and tensin homolog(PTEN)genes in SCID mice with prostate cancer orthotopic transplantation tumor.Methods Forty male SCID mice were randomly divided into model control group,low-dose group,medium-dose group and high-dose group according to the random number table method,with 10 mice in each group.Mouse model of prostate cancer orthotopic transplantation tumor was constructed by injecting human prostate cancer LNCaP cell line suspension into the dorsal lateral capsule of the prostate gland in SCID mice.After 2 weeks of modeling,the model control group was given 0.9％sodium chloride injection,the low-dose group was given 10 mg/kg icariin,the medium-dose group was given 40 mg/kg icariin,and the high-dose group was given 80 mg/kg icariin.Gavage treat-ment once a day for 5 weeks.Before and after 5 weeks of gavage treatment,5 mice in each group were taken and compared for tumor mass,tumor volume,and tumor inhibition rate.Flow cytometry was used to detect the cell cy-cle of LNCaP and calculate the ratio of each cycle.Real time quantitative PCR was used to detect the relative con-tent of PTEN mRNA in prostate tumor tissue.Results After treatment,tumor mass and volume in the model con-trol group were significantly increased compared to before treatment(P＜00.05).Tumor mass and volume in the medium-dose and high-dose groups were significantly reduced compared to before treatment(P＜0.05),and were significantly lower than those in the model control group(P＜0.05).The tumor inhibition rates in the medium-dose and high-dose groups were significantly higher than those in the low-dose group(P＜0.05).The S phase of tumor cells in the medium-dose and high-dose groups was significantly increased compared to before treatment(P＜0.05),and was significantly higher than that in the model control group(P＜0.05).The G0/G1 phase of tumor cells in the medium-dose and high-dose groups was significantly reduced compared to before treatment(P＜0.05),and was significantly lower than that in the model control group(P＜0.05).The relative content of PTEN mRNA in the medium-dose and high-dose groups was significantly increased compared to before treatment(P＜0.05),and was significantly higher than that in the model control group(P＜0.05).Conclusion Icariin might inhibit the pro-liferation of prostate cancer LNCaP cells by upregulate PTEN expression and alter cell cycle distribution,thereby effectively inhibit the growth of prostate cancer orthotopic transplantation tumor in SCID mice.
icariinprostate cancerphosphatase and tensin homologcell cycle
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