Clinical Study and Network Pharmacological Mechanism Exploration of Longqing Capsule in Treating Urinary Tract Infection
Objective To explore the therapeutic effect and mechanism of Longqing capsule in treating urinary tract infection through clinical study and network pharmacology.Methods Sixty female patients who were diag-nosed as simple urinary tract infection by western medicine and identified as dampness-heat in lower jiao syndrome by traditional Chinese medicine were selected and randomly divided into the control group and the trial group,with 30 cases in each group.Both groups were given oral treatment with cefaclor.The trial group received oral adminis-tration of Longqing capsule,while the control group received oral administration of Longqing capsule mimet-ics.After 7 days of treatment,the clinical symptom scores,quality of life scores,disappearance time of individual symptoms,total effective rate and safety indicators were compared in both groups.At the same time,the TCMSP database and Swiss Target Prediction server were used to obtain the active ingredients and potential targets of Longqing capsule,and the GeneCards database was used to obtain the relevant targets of urinary tract infec-tion.And the common targets were screened out,so as to construct the network of active ingredients-targets using Cytoscape.The PPI networks were constructed via the STRING database and KEGG and GO analyses were con-structed via the DAVID database.Results Compared with the control group,the urinary urgency syndrome scores,quality of life scores and disappearance time of individual symptoms in the trial group were decreased(all P＜0.05),while the total effective rate was increased(P＜0.05).The network pharmacology results showed that quercetin,kaempferol and other active ingredients were the main active ingredients of Longqing capsule against uri-nary tract infection.SRC,PIK3R1,HSP90AA1 and other genes were the core targets of Longqing capsule against urinary tract infection,which mainly enriched in cancer related pathways,MAPK signaling pathways,and other sig-naling pathways.Conclusion Longqing capsule may exert its anti-urinary tract infection effect through cancer re-lated pathways and other signaling pathways,which could relieve the symptoms of urinary frequency,urgency and urinary pain,and improve quality of life.
Longqing capsuleclinical studyurinary tract infectionnetwork pharmacologymechanism of action
万方数据
维普
