目的 探讨半枝莲碱A对EB病毒阳性弥漫性大B细胞淋巴瘤(EB virus-positive diffuse large B-cell lymphoma,EBV+DLBCL)细胞株Farage细胞增殖、凋亡及细胞周期的影响.方法 将Farage细胞分为对照组和不同浓度半枝莲碱A处理组,采用CCK-8法检测细胞增殖活性,流式细胞术检测细胞凋亡和细胞周期分布,RT-qPCR法检测半枝莲碱A作用于Farage细胞后细胞周期蛋白D3(cyclin D3)的mRNA表达以及 Western blot法检测cyclin D3蛋白表达.结果 CCK-8结果显示,与对照组相比,半枝莲碱A能够明显抑制Farage细胞增殖,差异有统计学意义(P＜0.05).流式细胞检测结果显示,半枝莲碱A诱导细胞凋亡呈剂量依赖性,且主要使细胞阻滞于G0/G1期.RT-qPCR和Western blot结果显示,半枝莲碱A以剂量依赖形式使Farage细胞cyclin D3的mRNA和蛋白表达减少,差异具有统计学意义(P均＜0.05).结论 半枝莲碱A具有抑制Farage细胞增殖并诱导其凋亡的作用,可使细胞阻滞于G0/G1期,并可能通过下调cyclin D3表达发挥抗肿瘤作用.
Effects of Scutebarbatine A on Proliferation,Apoptosis and Cell Cycle of EB Virus-positive Diffuse Large B-cell Lymphoma
Objective To investigate the effects of Scutebarbatine A on the proliferation,apoptosis and cell cy-cle of EB virus-positive diffuse large B-cell lymphoma(EBV+DLBCL)cell line Farage.Methods Farage cells were divided into the control group and groups treated with different concentrations of Scutebarbatine A.The cell prolif-eration activity was detected using CCK-8 method.Cell apoptosis and cell cycle distribution were detected by flow cytometry.The mRNA and protein expression of cyclin D3 were checked by RT-qPCR and Western blot after the action of Scutebarbatine A on Farage cells.Results The CCK-8 results showed that Scutebarbatine A was able to markedly inhibit the proliferation of Farage cells compared with the control group,and the difference was statisti-cally significant(P＜0.05).The flow cytometry results showed that Scutebarbatine A induced cell apoptosis in a dose-dependent manner and mainly blocked Farage cells in G0/G1 phase.RT-qPCR and Western blot results showed that Scutebarbatine A reduced the mRNA and protein expression of cyclin D3 in Farage cells in a dose-de-pendent manner,and the difference was statistically significant(all P＜0.05).Conclusion Scutebarbatine A could inhibit the proliferation of Farage cells and induce their apoptosis,which could block cells in G0/G1 phase and may exert anti-tumor effects by downregulating the expression of cyclin D3.
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