Objective To understand the current status of disinfection,sterilization and environmental hygiene in Stomatological Hospital Affiliated to Zunyi Medical University,to identify weak links and propose improvement measures,and to provide reference for nosocomial infection management.Methods Continuous monitoring of the disinfection quality was conducted for the clinical water,hand hygiene of medical personnel,surfaces,air,disinfection equipment,and in-use disinfectants in Stomatology Hospital Affiliated to Zunyi Medical University from 2020 to 2022.Sampling and inspection methods were referenced according to relevant standards such as the Disinfection Technical Specifications and the Hospital Disinfection Sanitation Standards.Statistical analysis was performed by SPSS 23.0 software,and Chi-square test was used to compare the difference between pass rates.P<0.05 was considered statistically significant.Results From 2020 to 2022,a total of 1 216 samples were collected and monitored,with an overall pass rate of 93.67%(1 139/1216).The pass rates for the three years were 95.09%(271/285),93.56%(334/357)and 93.03%(534/574),respectively,with no statistically significant differences in the pass rate(x2=1.368,P=0.505).Among the different monitoring items,the in-use disinfectants and object surfaces had higher pass rates(100.00%and 97.94%),while the clinical water and medical staff hand hygiene had relatively lower pass rates(90.24%and 85.77%).The comparison of pass rates among different monitoring items showed a statistically significant difference(x2=49.564,P<0.001).Only the clinical water demonstrated a significant trend of increasing pass rates over the three years(x2trend=8.528,P=0.014),while the other items did not show statistically significant differences in the pass rate over the three years.Conclusions From 2020 to 2022,the overall quality of disinfection,sterilization and environmental hygiene in Stomatological Hospital Affiliated to Zunyi Medical University was good.However,the pass rates for clinical water and medical staff hand hygiene were relatively low,indicating a potential risk of nosocomial infection.This requires proper identification and management.