Objective Four Yellow Qing Ling Water(SHQLY),a traditional Chinese medicine formula has been used for dry eye;However,anti-inflammatory activities of SHQYL are poorly understood.We elucidated the protective effect of SHQLY on the LPS-induced inflammation in RAW264.7 macrophage cells and to explore its potential mecha-nism.Methods RAW264.7 cells have been stimulated with LPS and treated with SHQLY.The cell viability of the LPS-treated RAW264.7 cells was examined by CCK-8 method.The modulation of SHQLY on anti-inflammation was detected by Elisa by measuring the release of inflammatory cytokines(TNF-α and IL-6),DCFH-DA fluorescence probe method was used to detect reactive oxygen species(ROS).Further,the content P-ERK/ERK,P-p38/p38,P-JNK/JNK,P-Iκβα/Iκβα and P-NF-κB/NF-KB in MAPKs/NF-κB signaling pathway were detected by western-blot method.The nuclear trans-location of NF-κB was detected by immunofluorescence using laser confocal scanning.Results Anti-inflammation assay demonstrated that SHQLY inhibited the production of the pro-inflammatory cytokines IL-6 and TNF-α in LPS-stimulated RAW264.7 macrophages.SHQLY decreased ROS accumulation.SHQLY down-regulated the protein levels of phospho-rylated proteins such as P-ERK,P-p38,P-JNK,P-IKβα and P-p65 in MAPKs/NF-KB pathway and inhibited NF-κB translocation into nucleus.Conclusion SHQLY protects LPS-induced inflammation in RAW264.7 cells,which may be re-lated to its inhibition of phosphorylation of MAPKs/NF-κB pathway.
维普
万方数据