中医眼耳鼻喉杂志2024，Vol.14Issue(3) ：124-126,148.DOI:10.3969/j.issn.1674-9006.2024.03.002

活血明目方对体外加压培养大鼠RGCs NF-κB信号通路凋亡相关因子Bax及Bcl-2的影响

Effects of blood-activating and eye-opening agents on apoptosis-related factors Bax and Bcl-2 of NF-κB signaling pathway in RGCs of rats cultured under pressure in vitro

赵颖 汪伟 母倩倩 蔡闻达 高佳
中医眼耳鼻喉杂志2024，Vol.14Issue(3) ：124-126,148.DOI:10.3969/j.issn.1674-9006.2024.03.002
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活血明目方对体外加压培养大鼠RGCs NF-κB信号通路凋亡相关因子Bax及Bcl-2的影响

Effects of blood-activating and eye-opening agents on apoptosis-related factors Bax and Bcl-2 of NF-κB signaling pathway in RGCs of rats cultured under pressure in vitro

赵颖 1汪伟 2母倩倩 1蔡闻达 2高佳3
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作者信息

  • 1. 650500,云南昆明,云南中医药大学;650504,云南昆明,昆明市中医医院
  • 2. 650504,云南昆明,昆明市中医医院
  • 3. 610075,四川成都,成都中医药大学眼科学院
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摘要

目的 观察活血明目方对体外加压大鼠视网膜神经节细胞(retinal ganglion cells,RGCs)中凋亡相关因子Bax和Bcl-2表达的干预作用.方法 将SD大鼠随机分为4组,每组3只大鼠,采取体外加压培养SD大鼠凋亡模型,制备活血明目方含药肠吸收液,观察活血明目方对体外加压RGCs在NF-κB信号通路作用下对Bax、Bcl-2表达的影响.结果 Q-PCR和Western blot检测发现,与模型组相比,含药肠吸收液组中Bax相对表达量显著下降(P＜0.05),而Bcl-2相对表达量则显著上升(P＜0.01).结论 活血明目方含药肠吸收液对RGCs凋亡具有一定的抑制作用.

Abstract

Objective To observe the intervention effect of blood-boosting and eye-opening agent on the expression of Bax and Bcl-2 genes in RGCs of rats pressurized in vitro,and to explore its mechanism of action.Methods SD rats were randomly divided into 4 groups of 3 rats each,and the apoptosis model of SD rats was cultured under in vitro pres-sure,and the intestinal absorption solution containing the medication of Live Blood and Mindfulness Formula was prepared to observe the effects of Live Blood and Mindfulness Formula on the expression of Bax and Bcl-2 by RGCs under the action of the NF-κB signalling pathway under in vitro pressure.Results Q-PCR and Western blot assays revealed that compared with the model group,the relative expression of Bax in the group containing the medicated intestinal absorption solution decreased significantly(P＜0.05),while the relative expression of Bcl-2 increased significantly(P＜0.01).Conclusion The blood-boosting and eye-opening agent can protect the visual function of rats pressurized in vitro and has a certain in-hibitory effect on optic nerve apoptosis.

关键词

RGCs/中药/活血明目剂/体外加压培养/NF-κB信号通路/Bax/Bcl-2

Key words

RGCs/Traditional Chinese medicine/blood-activating and eye-opening agents/In vitro pressurized culture/NF-κB signaling pathway/Bax/Bcl-2

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基金项目

云南省科技厅中医联合专项面上项目(202101AZ070001-090)

云南省科技厅中医联合专项面上项目(202301AZ070001-100)

昆明市卫健委卫生科研课题(2021-08-01-001)

出版年

2024
中医眼耳鼻喉杂志
成都中医药大学

中医眼耳鼻喉杂志

ISSN：1674-9006
参考文献量15
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