Genetic Analysis of Lepidium meyenii Walp.Resources from Different Habitats Based on ISSR Markers
Objective:To study the population genetic diversity and relationships of Lepidium meyenii Walp.resources from 5 different habitats according to their DNA molecule among them.Methods:Inter-simple sequence repeat (ISSR)molecular marker technique was adopted to analyze genetic diversity of L.meyenii from different habitats.8 ISSR primers with good repeatability screened from 20 ISSR primers were screened out with PCR and the amplification products were examined by 1.5％ agarose gel electrophoresis.Genetic parameters were analyzed statistical and population genetic cluster was assessed by using UPGMA method (NTSYS software).Results:A total of 51 bands were amplified from 8 ISSR primers,of which 22 bands were polymorphic loci.The percentage of polymorphic fragment was up to 43.1％.The genetic similarity coefficient of the l lsamples was 0.627 5-0.960 8 and samples could be divided into 2 categories differencing between Peru and China.However,9 samples from China could not be distinguished from each other significantly through cluster analysis.Conclusion:The ISSR markers can be used for identification of L.meyenii from Peru or China,but the genetic variation of Chinese L.meyenii is small with strong genetic stability.
国家科技期刊平台
NSTL
万方数据
