Antibacterial Activity and Mechanism of Essential Oil from Cinamomum camphora on Staphylococcus aureus
Objective:To investigate the antibacterial activity and mechanism of the essential oil from Cinamomum camphora on Staphylococcus aureus.Methods:The inhibition zone experiment and tube double dilution method were used to determine the essential oil's antimicrobial activity and the minimum inhibitory concentration(MIC).The antibacterial mechanism experiments were carried out by determining changes of reducing sugar content,protein content and electric conductivity.Results:The MIC to S.aureus was 12.5%.The essential oil could affect the membrane permeability of S.aureus.After treated with the essential oil for 4 hours,the reducing sugar content of the experimental groups was increased by 25.48%,31.02%,31.25%(P<0.05)than that of the control group,respectively,and the total content of proteins increased by 26.86%,31.43%,31.23%(P<0.05),respectively.The difference was significant.Conclusion:The essential oil showed obvious antibacterial activity against S.aureus.The antimicrobial mechanism is inferred that bacterial cell structure is damaged,cell membrane permeability increased.
Essential oilantimicrobial activitiesantibacterial mechanisminhibition zone diameters.
国家科技期刊平台
NSTL
万方数据
维普
