Simultaneous Determination of Epicatechin and 4β-2(aminoethylthio)Epicatechin in Dog Plasma by UPLC-MS/MS
Objective:To establish a sensitive and rapid liquid chromatography-tandem mass spectrometry(LC-MS/MS)method to simultaneously determinate epicatechin(EC)and 4β-2(aminoethylthio)epicatechin(Cya-EC)in plasma of beagle dogs after oral administration of WeiMaiNing capsule.Method:Chromatographic separation was achieved on an Waters BEH Shield RP18(100 mm×2.1 mm,1.7 μm)using a step gradient program with the mobile phase of 0.1% formic acid aqueous solution and acetonitrile with 0.1% formic acid,at a flow-rate of 0.30 mL-1.Electrospray ionization(ESI)source was applied and operated in the positive ion mode.Multiple reaction monitoring mode with the transitions of m/z 291.1→138.9(EC),m/z 366.1→288.9(Cya-EC)and m/z 260.2→116.3(IS,propranolol)was performed to quantify,respectively.Results:The linear concentration ranges of calibration curves for EC and Cya-EC were 10-2000 ng·mL-1.The inter-or intra-day precision(RSD)was less than 14.82%,and the accuracy(RE)was within±4.86%.The pharmacokinetic parameters were calculated by DAS3.0.The t1/2 of EC and Cya-EC were 66.3 and 90.4 h.And the AUC0-∞ of EC and Cya-EC were 2 114.7 and 12 909.5 ng·h·mL-1 in beagle dog plasma after oral administration of WeiMaiNing capsules.Conclusion:The method was sensitive,rapid,convenient,using less plasma and was proved to be suitable for preclinical pharmacokinetic studies of EC and Cya-EC in beagle dog after oral administration of Wei MaiNing capsules.
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万方数据
维普
