首页|蓝莓果实中花青素类成分的超声波提取工艺研究

蓝莓果实中花青素类成分的超声波提取工艺研究

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  • 国家科技期刊平台
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目的:研究超声波提取法提取蓝莓中花青素类成分的工艺方法.方法:采用正交试验,选用L9(34)正交表考察超声波提取法提取时,提取液的甲醇比例、提取液的盐酸含量、提取时间和料液比对蓝莓中花青素类成分提取率的影响.花青素的总含量用HPLC法测定.结果:超声波提取法提取花青素类成分影响因素的大小顺序为盐酸含量＞甲醇比例＞提取时间＞料液比,最佳提取工艺条件为提取液80％甲醇-水溶液,提取液含盐酸0.2％,料液比1∶25(g∶ mL),提取时间50 min.结论:采用优化的工艺条件稳定可行,蓝莓中花青素类成分的含量为5.12 mg·g-1.
Study on Ultrasonic Extraction of Anthocyanins from Blueberry Fruit
Objective:To study the method of extraction of anthocyanins from blueberry by ultrasonic extraction.Methods:Taking the ratio of methanol solvent,the ratio of added hydrochloric acid,the time of extraction and liquid to feed ratio as indexes,L9 (34) orthogonal test was adopted to investigate the extraction of blueberry anthocyanins.The total content of anthocyanins was determined by HPLC.Results:The order of the influencing factors of ultrasonic extraction of anthocyanins from high to low was as follows:the content of hydrochloric acid,the ratio of methanol and the extraction time and liquid to feed ratio.Optimal ultrasonic extraction conditions were as follow:80％ methanol-water as solvent which contained 0.2％ hydrochloric acid,liquid to feed ratio was 25∶1 (mL∶g),50 min per time.Conclusion:The developed extraction process was stable and feasible,the content of anthocyanins in blueberry fruit was 5.12 mg ·g-1.

Blueberryanthocyaninultrasonic extractionorthogonal test

杨莉、李珂珂、李沙沙、陈书笑、陶丽、弓晓杰

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大连大学环境与化学工程学院,辽宁 大连116622

大连大学医学院,辽宁 大连116622

蓝莓 花青素 超声波提取 正交试验

辽宁省科技厅科学技术计划项目辽宁省优秀人才支持计划辽宁省自然科学基金国家自然科学基金

2014204007LR2013058201502065781603272

2017

10.13313/j.issn.1673-4890.2017.7.022

中国现代中药
中国中药协会,中国医药集团总公司,中国药材公司

中国现代中药

CSTPCD
影响因子：0.65
ISSN：1673-4890
年,卷(期)：2017.19(7)
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