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陕产宽叶重楼的生药学研究

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目的:研究陕产宽叶重楼根茎的生药学特征.方法:采用形态组织学鉴别方法, 研究原植物形态、生药性状和显微特征, 并使用HPLC法测定4种重楼皂苷的总含量.结果:陕产宽叶重楼原植物来源清楚.根茎显微特征:横切面有栓化表皮, 皮层宽广、内皮层不连续, 中柱为薄壁组织中散布周木式维管束等结构;生药粉末中含极多单粒淀粉, 常见草酸钙针晶、环纹和螺纹导管.HPLC法测定重楼皂苷I、Ⅱ、Ⅵ、Ⅶ总含量达到3%以上.结论:陕产宽叶重楼来源确定、生药性状稳定, 具有特定显微结构, 重楼皂苷I、Ⅱ、Ⅵ、Ⅶ总含量均达到了2015版《中华人民共和国药典》标准.该研究阐释了陕西民间将宽叶重楼根茎与药典所载中药重楼同名等效使用的合理性, 为制订宽叶重楼质量标准、寻求中药重楼替代资源奠定了基础.
Pharmacognostical Studies of Paris polyphylla var. latifolia from Shaanxi Province
Objective:To research the pharmacognostical characteristics of crude medicine of Parispolyphylla var.latifolia from Shaanxi Province.Methods:The morphology, pharmacognosy and microscopic characteristics of the rhizome of P.polyphylla var.latifolia from Shaanxi Province were studied by morphology and histology identification.And HPLC was adopted in the content determination of the total content of 4 saponins (polyphyllinsⅠ, Ⅱ, Ⅵ, Ⅶ).Results:The plant origin of P.polyphylla var.latifolia from Shaanxi Province was explicited.The microscopic characteristics of the rhizome as follows:In the transection section, suberification epidermis, wide cortex and discontinuous endodermis, and some stele of amphivasal vascular bundle in the parenchyma tissue were observed.And a great many simple starch grains were observed in the powder, common calcium oxialate acicular crystal, annular vessel and spiral vessel.In addition, the total content of 4 saponins (polyphyllinsⅠ, Ⅱ, Ⅵ, Ⅶ) reached 3.0%.Conclusion:P.polyphylla var.latifolia from Shaanxi Province have definite origin sources, stable pharmacognostical characteristics and specific microstructures, and content of 4 saponins (polyphyllinsⅠ, Ⅱ, Ⅵ, Ⅶ) met the requirement of Chinese Pharmacopoeia (2015).The results explain that P.polyphylla var.latifolia is used as Paridis Rhizoma recorded in the Chinese Pharmacopoeia (2015) is reasonable, and may lay a foundation for formulating the quality standard of crude rhizome medicine of P.polyphylla var.latifolia.

Paris polyphylla Sm.var.latifolia Wang et Chang from Shaanxi Provincepharmacognostical characteristicsmicroscopic structurepolyphyllinsalternative sources of Paridis Rhizomaquality standard

程虎印、程江雪、王艳、张景霞、颜永刚、彭亮、杨新杰、高静

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陕西中医药大学,陕西 西安 712046

陕西省微生物研究所,陕西 西安 710043

陕产宽叶重楼 生药学特征 显微特征 重楼皂苷 重楼替代资源 质量标准

陕西省教育厅2012年省级重点实验室科研项目陕西省社会发展科技攻关项目

12JSO402016SF—360

2019

中国现代中药
中国中药协会,中国医药集团总公司,中国药材公司

中国现代中药

CSTPCD
影响因子:0.65
ISSN:1673-4890
年,卷(期):2019.21(2)
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