Quality Markers of Cistanches Herba Based on Antioxidant Spectrum-effect Relationship
Objective:To establish a research method for the quality markers(Q-markers)of Cistanches Herba(CH)based on the antioxidant spectrum-effect relationship,providing a basis for the study of its pharmacological substances and quality control.Methods:Ultra-high-performance liquid chromatography(UPLC)was used to establish fingerprint profiles for 10 batches of CH.A cell oxidative damage model was established using H2O2,and the antioxidant capacity of CH was determined based on the half-maximal inhibitory concentration of reactive oxygen species,as well as the levels of superoxide dismutase and catalase as pharmacological indicators.The spectrum-effect relationship was constructed through principal component analysis,Pearson correlation analysis,and partial least squares regression analysis.The active components were subjected to pharmacological validation.Results:Fingerprint profiles for CH were established using UPLC,identifying 17 common peaks.Based on spectrum-effect correlation analysis,nine components were selected.The pharmacological validation results indicated that geniposidic acid,8-epiloganic acid,echinacoside,acteoside,isoacteoside,and tubuloside A exhibited antioxidant activity in a concentration-dependent manner.Conclusion:This study explored Q-markers for CH based on the spectrum-effect relationship,providing reference information for its pharmacological substance foundation and quality standard improvement.
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