Evaluation of Tingli Dazao Xiefei Decoction Based on HPLC Fingerprint Technology
Objective:To establish the high-performance liquid chromatography(HPLC)fingerprint of Tingli Dazao Xiefei Decoction and a method for determination of six index components.Method:The HPLC fingerprints of 14 batches of Tingli Dazao Xiefei Decoction were established by reverse-phase HPLC gradient elution method according to the similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine(Version 2012A).At the same time,the contents of six index components(quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside,rutin,isoquercitrin,isorhamnetin-3-O-β-D-glucoside,quercetin and isorhamnetin)were determined.Results:A total of 27 common peaks were calibrated according to the HPLC characteristic fingerprints of Tingli Dazao Xiefei Decoction,and six chromatographic peaks were identified,including peak 5(quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside),peak 18(rutin),peak 19(isoquercitrin),peak 21(isorhamnetin-3-O-β-D-glucose),peak 24(quercetin),and peak 27(isorhamnetin).The content determination for the 14 batches of Tengli Dazao Xiefei Decoction resulted in the following mass concentrations.The content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside was 111.33-118.71 μg·mL-1,the content of rutin was 9.16-9.36 μg·mL-1,the content of isoquercitrin was 16.51-17.26 μg·mL-1,the content of isorhamnetin-3-O-β-D-glucoside was 12.23-12.62 μg·mL-1,and the content of quercetin was 3.10-3.36 μg·mL-1,and the content of isorhamnetin was 3.51-3.62 μg·mL-1.The established fingerprint and content determination method exhibited good precision,stability and reproducibility.Conclusion:The established HPLC fingerprint profile and multi-index component quantification method are stable and reliable,which can provide a reference for the study on the material basis and quality standard of Tingli Dazao Xiefei Decoction.
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