摘要
目的:建立葶苈大枣泻肺汤物质基准的高效液相色谱法(HPLC)指纹图谱和 6 个指标成分的含量测定方法.方法:采用反相高效液相色谱梯度洗脱法,借助"中药色谱指纹图谱相似度评价系统"(2012A版)建立 14 批葶苈大枣泻肺汤的HPLC特征指纹图谱,同时测定槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷、芦丁、异槲皮苷、异鼠李素-3-O-β-D-葡萄糖苷、槲皮素和异鼠李素 6 个指标成分的含量.结果:葶苈大枣泻肺汤的HPLC特征指纹图谱共标定了 27 个共有峰,6 个特征峰分别为 5 号峰(槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷)、18 号峰(芦丁)、19 号峰(异槲皮苷)、21 号峰(异鼠李素-3-O-β-D-葡萄糖)、24 号峰(槲皮素)和 27 号峰(异鼠李素).对 14 批葶苈大枣泻肺汤进行含量测定,槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷、芦丁、异槲皮苷、异鼠李素-3-O-β-D-葡萄糖苷、槲皮素和异鼠李素质量浓度分别为 111.33～118.71、9.16～9.36、16.51～17.26、12.23～12.62、3.10～3.36、3.51～3.62 μg·mL-1,方法精密度、稳定性、重复性均良好.结论:建立的HPLC指纹图谱和多指标成分含量测定方法稳定可靠,可为葶苈大枣泻肺汤的质量标准研究提供参考.
Abstract
Objective:To establish the high-performance liquid chromatography(HPLC)fingerprint of Tingli Dazao Xiefei Decoction and a method for determination of six index components.Method:The HPLC fingerprints of 14 batches of Tingli Dazao Xiefei Decoction were established by reverse-phase HPLC gradient elution method according to the similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine(Version 2012A).At the same time,the contents of six index components(quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside,rutin,isoquercitrin,isorhamnetin-3-O-β-D-glucoside,quercetin and isorhamnetin)were determined.Results:A total of 27 common peaks were calibrated according to the HPLC characteristic fingerprints of Tingli Dazao Xiefei Decoction,and six chromatographic peaks were identified,including peak 5(quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside),peak 18(rutin),peak 19(isoquercitrin),peak 21(isorhamnetin-3-O-β-D-glucose),peak 24(quercetin),and peak 27(isorhamnetin).The content determination for the 14 batches of Tengli Dazao Xiefei Decoction resulted in the following mass concentrations.The content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside was 111.33-118.71 μg·mL-1,the content of rutin was 9.16-9.36 μg·mL-1,the content of isoquercitrin was 16.51-17.26 μg·mL-1,the content of isorhamnetin-3-O-β-D-glucoside was 12.23-12.62 μg·mL-1,and the content of quercetin was 3.10-3.36 μg·mL-1,and the content of isorhamnetin was 3.51-3.62 μg·mL-1.The established fingerprint and content determination method exhibited good precision,stability and reproducibility.Conclusion:The established HPLC fingerprint profile and multi-index component quantification method are stable and reliable,which can provide a reference for the study on the material basis and quality standard of Tingli Dazao Xiefei Decoction.
基金项目
山东省重大科技创新工程项目(2018CXGC1305)
山东省中医经典名方协同创新中心开放课题(2018KFY02)
国家科技期刊平台
NSTL
万方数据