Research on the Processing Technology and Quality Evaluation Methods of Bufonis Oviductus
Objective:To optimize the drying process technology of Bufonis Oviductus,and establish a quality evaluation method based on high-performance liquid chromatography(HPLC)fingerprint and amino acid content determination.Methods:Amino acids and extracts were used as indicators in the optimization of hot-air-drying,nature-shadow-drying,freeze-drying and vacuum-drying for Bufonis Oviductus.The impacts of several factors on the Bufonis oviductus were investigated including,heating temperature,heating time,material thickness,pre-freezing time,freeze-drying time,vacuum drying temperature,and vacuum drying time.The fingerprints of 15 batches samples were established by HPLC.Common peaks were determined and their similarities were evaluated.Pre-column derivatization was also used to determine the content of amino acids.Results:The optimum conditions for processing Bufonis Oviductus were determined as hot-air-drying at 50 ℃ for 11 h with the samples placed in a single layer.A total of 7 common peaks were identified in the HPLC fingerprint of Bufonis Oviductus,and the similarities of 15 samples were greater than 0.99.The contents of isoleucine,leucine,phenylalanine and lysine are 0.40%-0.57%,0.57%-0.78%,0.54%-0.75%,0.26%-0.86%,respectively.Conclusion:The determined drying process of Bufonis Oviductus as well as quality evaluation methods are simple and reproducible,which can provide a reference for the processing and quality evaluation of Bufonis Oviductus.
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