基于薄层色谱与一测多评法的沙棘颗粒质量评价方法研究
Quality Evaluation of Shaji Granules Based on Thin Layer Chromatography and Quantitative Analysis of Multi-components by A Single Marker
樊修和 1唐志书 2宋忠兴 1王昌利 3王珂1
作者信息
- 1. 陕西中医药大学 陕西中药资源产业化省部共建协同创新中心,陕西 咸阳 712046
- 2. 陕西中医药大学 陕西中药资源产业化省部共建协同创新中心,陕西 咸阳 712046;中国中医科学院,北京 100700
- 3. 陕西海天制药有限公司,陕西 咸阳 712000
- 折叠
摘要
目的:优化中成药沙棘颗粒的薄层鉴别方法,建立多成分高效液相色谱一测多评方法.方法:采用薄层色谱法对槲皮素、异鼠李素和山柰素进行定性鉴别;采用高效液相色谱法,C18色谱柱(250 mm×4.6 mm,5 µm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速为 1.0 mL·min-1,柱温为 35℃,检测波长为 365 nm,以槲皮素为内参物计算异鼠李素和山柰素含量.结果:薄层色谱鉴别方法斑点清晰、专属性好;与外标法对比,一测多评法准确度高、耐用性好.结论:优化的薄层色谱法与建立的一测多评法可靠、准确、重复性好,可为沙棘颗粒的质量标准提升提供参考.
Abstract
Objective:To optimize the thin layer chromatography(TLC)and establish a quantitative analysis of multi-components by a single marker(QAMS)method for Shaji Granules.Methods:Quercetin,isorhamnetin and kaempferol were qualitatively identified by TLC.A high-performance liquid chromatography(HPLC)was established using C18 column(250 mm×4.6 mm,5 µm)with acetonitrile-0.1%phosphoric acid solution as mobile phase in gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was 35℃,and the detection wavelength was 365 nm.Isorhamnetin and kaempferol were determined with quercetin as internal reference.Results:TLC had good specificity and the spots were clear.Compared with the external standard method,the QAMS method was accurate and feasible.Conclusion:The optimized TLC and the established QAMS method were reliable,accurate and reproducible,which could provide a comprehensive reference for improving the quality control standards of Shaji Granules.
关键词
沙棘颗粒/槲皮素/异鼠李素/山柰素/薄层色谱法/一测多评法Key words
Shaji Granules/quercetin/isorhamnetin/kaempferol/thin layer chromatography(TLC)/quantitative analysis of multi-components by a single marker(QAMS)引用本文复制引用
基金项目
陕西省重点研发计划(202190025)
内蒙古自治区科技重大专项(2019)(2019ZD005)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据