摘要
目的:建立复方罗布麻颗粒的超高效液相色谱法(UPLC)特征图谱,同时对新绿原酸、绿原酸、隐绿原酸、金丝桃苷、异槲皮苷、木犀草苷进行含量测定.方法:以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,色谱柱为Waters CORTECS T3(100 mm×2.1 mm,1.6 μm),流速为 0.33 mL·min-1,检测波长为 360 nm,柱温为30℃.结果:得到 15 批复方罗布麻颗粒特征图谱,确定特征峰 14 个,相似度值均大于 0.95.新绿原酸、绿原酸、隐绿原酸、金丝桃苷、异槲皮苷、木犀草苷分别在相应质量浓度范围内与其峰面积呈良好线性关系(r≥0.999 9);平均回收率分别为 99.9%、100.8%、99.9%、97.6%、101.1%、100.6%,RSD均小于 2.0%.结论:该方法具有较高的灵敏度、良好的重复性,可为科学、全面地评价和控制复方罗布麻颗粒的质量提供参考.
Abstract
Objective:To establish a ultra performance liquid chromatography(UPLC)for Compound Luobuma Granules,and neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,hyperoside,isoquercitrin and cynaroside were determined.Methods:Gradient elution was used with Waters CORTECS T3(100 mm×2.1 mm,1.6 μm)column and acetonitrile-0.1%phosphoric acid as mobile phase at a flow rate of 0.33 mL·min-1.The detection wavelength was 360 nm,and column temperature was 30℃.Results:The specific chromatogram of 15 batches of Compound Luobuma Granules were established,from which 14 characteristic peaks were identified,with all the similarities more than 0.95.Neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,hyperoside,isoquercitrin and cynaroside all had good linear relationships with the peak area in their corresponding ranges(r≥0.999 9).The average recoveries of the six components were 99.9%,100.8%,99.9%,97.6%,101.1%,and 100.6%,respectively,with RSD<2.0%.Conclusion:This developed UPLC had high sensitivity and good reproducibility,which provided a basis for scientific evaluation and comprehensive control of the quality of Compound Luobuma Granules.
基金项目
工信部产业技术基础公共服务平台项目(2022)(2022-230-221)
国家科技期刊平台
NSTL
维普
万方数据