基于UHPLC-Q-Exactive Orbitrap MS的复方一枝蒿颗粒化学成分快速鉴定
Rapid Identification of Chemical Components in Composite Yizhihao Granules Based on UHPLC-Q-Exactive Orbitrap MS
刘明娟 1李凯琳 1张敏 1唐苏女 1刘玲 1李晴 1郭俐兰 1杨建波 2李辉1
作者信息
- 1. 湖南医药学院 药学院 侗医药研究湖南省重点实验室,湖南 怀化 418000
- 2. 新疆维吾尔自治区药品检验研究院 国家药品监督管理局中药(维药)质量控制重点实验室,新疆 乌鲁木齐 830054;中国食品药品检定研究院,北京 100050
- 折叠
摘要
目的:采用超高效液相色谱-四极杆-静电轨道场离子阱质谱法对复方一枝蒿颗粒的化学成分进行分析.方法:采用Thermo Scientific Syncronis C18 色谱柱(100 mm×2.1 mm,1.7 μm),流动相为 0.1%甲酸-乙腈,梯度洗脱,流速为 0.3 mL·min-1,柱温为 45℃,进样量为 2 μL.在正负离子模式下采集复方一枝蒿颗粒的质谱数据,根据高分辨质谱提供的精确相对分子质量和碎片离子信息,结合对照品比对和相关文献报道对复方一枝蒿颗粒的化学成分进行分析.结果:共分析鉴定出 90 个化合物,包括 2 个氨基酸类、8 个萜类、25 个有机酸类、11 个含氮类、44 个黄酮类成分.其中,16 个成分通过与对照品比对确认.结论:分析鉴定了复方一枝蒿颗粒的化学成分,为其质量控制及药效物质基础研究提供了依据.
Abstract
Objective:The chemical components in Composite Yizhihao Granules were analyzed using UHPLC-Q-Exactive Orbitrap MS.Methods:Thermo Scientific Syncronis C18 column(100 mm×2.1 mm,1.7 μm)was used with a mobile phase of 0.1%formic acid acetonitrile and a gradient elution at the flow rate of 0.3 mL·min-1.The column temperature was maintained at 45℃,and the injection volume was 2 μL.The mass spectrometry data of Composite Yizhihao Granules were collected in positive and negative ion mode.According to the accurate molecular weight and fragment ion information provided by the high-resolution mass spectrometry,the chemical components of Composite Yizhihao Granules were identified and analyzed in combination with the comparison of standard products and relevant literature reports.Results:A total of 90 compounds were identified and analyzed,including 2 amino acids,8 terpenes,25 organic acids,11 nitrogen-containing compounds,and 44 flavonoids.Among them,16 components were confirmed by comparison with the reference standards.Conclusion:In this study,the chemical components of Composite Yizhihao Granules were analyzed and identified,providing a basis for its quality control and the study of its pharmacological substances.
关键词
复方一枝蒿颗粒/超高效液相色谱-四极杆-静电轨道场离子阱质谱法/化学成分Key words
Composite Yizhihao Granules/UHPLC-Q-Exactive Orbitrap MS/chemical components引用本文复制引用
基金项目
新疆维吾尔自治区"2+5"重点人才计划(第二批)(2023)()
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据