Comprehensive Quality Evaluation of Xianshi Lotion Based on Multi-component Quantitative Combined with PCA,OPLS-DA and EW-TOPSIS Analysis
Objective:To establish a multi-component quantitative detection method of Xianshi Lotion,principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),combined with entropy weight-technique for order preference by similarity to ideal solution(EW-TOPSIS)method was applied to analyze the differences in the results of multi-component content and evaluate its comprehensive quality.Methods:HPLC was used with Global Chromatography C18 column as the chromatographic column and acetonitrile-0.1%phosphoric acid as the mobile phase.The contents of limonin,evodiamine,rutaecarpine,imperatorin,osthole,isoimperatorin,cimifugin,5-O-methylvisammioside,pseudolaric acid C,pseudolaric acid B,hydroxy-α-sanshool,hydroxy-β-sanshool and hydroxy-γ-sanshool in 12 batches of Xianshi Lotion were determined.The quantitative results were analyzed by chemometrics and EW-TOPSIS.Results:The 13 components had good linear relationships within their respective ranges(r>0.999),and the average recovery was 96.90%-100.09%(n=9,RSD<2.0%).The results of PCA showed that 12 batches of Xianshi Lotion were divided into 3 groups.The results of OPLS-DA showed that osthole,pseudolaric acid B,hydroxy-α-sanshool,cimifugin,limonin and imperatorin were the differential characteristic markers of Xianshi Lotion.The EW-TOPSIS analysis results showed that the Euclid approach degree Ci value was between 0.188 7-0.692 0.There were some differences in the content of 13 components in products between different enterprises.Conclusion:The established method is convenient and accurate,and can be used for the comprehensive evaluation of the quality of Xianshi Lotion.The multi-component quantitative combined with PCA,OPLS-DA and EW-TOPSIS methods can also be used for the detection and quality evaluation of other traditional Chinese medicine preparations.
国家科技期刊平台
NSTL
万方数据
维普
