Studies on Neuroprotective Effects of 3,6'-Disinapoyl Sucrose
Objective:To study the neuroprotective effects of 3,6'-disinapoyl sucrose(DISS),an oligosaccharide ester component of the traditional Chinese medicine Polygala tenuifolia Willd.Methods:Different concentrations of DISS were co-incubated with APP-SH-SY5Y cells for 24 h.The survival rate was detected by methyl thiazole tetrazolium(MTT)assay,The intracellular reactive oxygen species(ROS)levels were detected by immunofluorescence.The activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were detected by biochemical methods.The intracellular and culture medium contents of β-amyloid protein(Aβ)level were detected by enzyme-linked immunosorbent assay(ELISA).The expression of amyloid precursor protein(APP),microtubule-associated protein lightchain 3-Ⅰ(LC3-Ⅰ)and LC3-Ⅱ in cells were detected by western blotting.Results:DISS could increase the viability of APP-SH-SY5Y cells(P<0.05),decrease the production of intracellular ROS(P<0.01),and increase the activities of SOD and GSH-px in APP-SH-SY5Y cells(P<0.05).Meanwhile,DISS also decreased the Aβ content in APP-SH-SY5Y cells(P<0.01)and cell culture medium(P<0.05),significantly increased the expression level of intracellular LC3Ⅱ protein and increased the ratio of LC3Ⅱ/LC3 Ⅰ(P<0.05).However,DISS had no significant effect on APP expression.Conclusion:DISS may exert neuroprotective effects by reducing intracellular oxidative stress,decreasing cellular Aβ expression and regulating autophagy.
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